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Open AccessArticle

A Disposable Passive Microfluidic Device for Cell Culturing

1
Department of Experimental and Clinical Medicine, University of Catanzaro, Germaneto, 88100 Catanzaro, Italy
2
Department of Chemical and Biochemical Engineering, Technology University of Denmark, 2800 Kongens Lyngby, Denmark
3
Physical Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia
*
Author to whom correspondence should be addressed.
Biosensors 2020, 10(3), 18; https://doi.org/10.3390/bios10030018
Received: 14 January 2020 / Revised: 21 February 2020 / Accepted: 26 February 2020 / Published: 29 February 2020
In this work, a disposable passive microfluidic device for cell culturing that does not require any additional/external pressure sources is introduced. By regulating the height of fluidic columns and the aperture and closure of the source wells, the device can provide different media and/or drug flows, thereby allowing different flow patterns with respect to time. The device is made of two Polymethylmethacrylate (PMMA) layers fabricated by micro-milling and solvent assisted bonding and allows us to ensure a flow rate of 18.6 μL/h - 7%/day, due to a decrease of the fluid height while the liquid is driven from the reservoirs into the channels. Simulations and experiments were conducted to characterize flows and diffusion in the culture chamber. Melanoma tumor cells were used to test the device and carry out cell culturing experiments for 48 h. Moreover, HeLa, Jurkat, A549 and HEK293T cell lines were cultivated successfully inside the microfluidic device for 72 h. View Full-Text
Keywords: passive microfluidic device; cell culture; micro-bioreactor passive microfluidic device; cell culture; micro-bioreactor
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Guzzi, F.; Candeloro, P.; Coluccio, M.L.; Cristiani, C.M.; Parrotta, E.I.; Scaramuzzino, L.; Scalise, S.; Dattola, E.; D’Attimo, M.A.; Cuda, G.; Lamanna, E.; Passacatini, L.C.; Carbone, E.; Krühne, U.; Di Fabrizio, E.; Perozziello, G. A Disposable Passive Microfluidic Device for Cell Culturing. Biosensors 2020, 10, 18.

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