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A Disposable Passive Microfluidic Device for Cell Culturing

Department of Experimental and Clinical Medicine, University of Catanzaro, Germaneto, 88100 Catanzaro, Italy
Department of Chemical and Biochemical Engineering, Technology University of Denmark, 2800 Kongens Lyngby, Denmark
Physical Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia
Author to whom correspondence should be addressed.
Biosensors 2020, 10(3), 18;
Received: 14 January 2020 / Revised: 21 February 2020 / Accepted: 26 February 2020 / Published: 29 February 2020
In this work, a disposable passive microfluidic device for cell culturing that does not require any additional/external pressure sources is introduced. By regulating the height of fluidic columns and the aperture and closure of the source wells, the device can provide different media and/or drug flows, thereby allowing different flow patterns with respect to time. The device is made of two Polymethylmethacrylate (PMMA) layers fabricated by micro-milling and solvent assisted bonding and allows us to ensure a flow rate of 18.6 μL/h - 7%/day, due to a decrease of the fluid height while the liquid is driven from the reservoirs into the channels. Simulations and experiments were conducted to characterize flows and diffusion in the culture chamber. Melanoma tumor cells were used to test the device and carry out cell culturing experiments for 48 h. Moreover, HeLa, Jurkat, A549 and HEK293T cell lines were cultivated successfully inside the microfluidic device for 72 h. View Full-Text
Keywords: passive microfluidic device; cell culture; micro-bioreactor passive microfluidic device; cell culture; micro-bioreactor
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MDPI and ACS Style

Guzzi, F.; Candeloro, P.; Coluccio, M.L.; Cristiani, C.M.; Parrotta, E.I.; Scaramuzzino, L.; Scalise, S.; Dattola, E.; D’Attimo, M.A.; Cuda, G.; Lamanna, E.; Passacatini, L.C.; Carbone, E.; Krühne, U.; Di Fabrizio, E.; Perozziello, G. A Disposable Passive Microfluidic Device for Cell Culturing. Biosensors 2020, 10, 18.

AMA Style

Guzzi F, Candeloro P, Coluccio ML, Cristiani CM, Parrotta EI, Scaramuzzino L, Scalise S, Dattola E, D’Attimo MA, Cuda G, Lamanna E, Passacatini LC, Carbone E, Krühne U, Di Fabrizio E, Perozziello G. A Disposable Passive Microfluidic Device for Cell Culturing. Biosensors. 2020; 10(3):18.

Chicago/Turabian Style

Guzzi, Francesco, Patrizio Candeloro, Maria L. Coluccio, Costanza M. Cristiani, Elvira I. Parrotta, Luana Scaramuzzino, Stefania Scalise, Elisabetta Dattola, Maria A. D’Attimo, Giovanni Cuda, Ernesto Lamanna, Lucia C. Passacatini, Ennio Carbone, Ulrich Krühne, Enzo Di Fabrizio, and Gerardo Perozziello. 2020. "A Disposable Passive Microfluidic Device for Cell Culturing" Biosensors 10, no. 3: 18.

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