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Open AccessArticle

Design of Multiplex Lateral Flow Tests: A Case Study for Simultaneous Detection of Three Antibiotics

A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky prospect 33, Moscow 119071, Russia
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Biosensors 2020, 10(3), 17; https://doi.org/10.3390/bios10030017
Received: 31 December 2019 / Revised: 11 February 2020 / Accepted: 21 February 2020 / Published: 27 February 2020
The presented study is focused on the impact of binding zone location on immunochromatographic test strips on the analytical parameters of multiplex lateral flow assays. Due to non-equilibrium conditions for such assays the duration of immune reactions influences significantly the analytical parameters, and the integration of several analytes into one multiplex strip may cause an essential decrease of sensitivity. To choose the best location for binding zones, we have tested reactants for immunochromatographic assays of lincomycin, chloramphenicol, and tetracycline. The influence of the distance to the binding zones on the intensity of coloration and limit of detection (LOD) was rather different. Basing on the data obtained, the best order of binding zones was chosen. In comparison with non-optimal location the LODs were 5–10 fold improved. The final assay provides LODs 0.4, 0.4 and 1.0 ng/mL for lincomycin, chloramphenicol, and tetracycline, respectively. The proposed approach can be applied for multiplexed assays of other analytes. View Full-Text
Keywords: multiparametric assay; rapid tests; immunochromatography; antibiotics; non-equilibrium interactions multiparametric assay; rapid tests; immunochromatography; antibiotics; non-equilibrium interactions
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Bartosh, A.V.; Sotnikov, D.V.; Hendrickson, O.D.; Zherdev, A.V.; Dzantiev, B.B. Design of Multiplex Lateral Flow Tests: A Case Study for Simultaneous Detection of Three Antibiotics. Biosensors 2020, 10, 17.

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