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Open AccessArticle

Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone

1
Department of Internal Medicine, Division of Cardiology, Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung 813, Taiwan
2
Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan
3
Department of Bioengineering, Imperial College, London SW7 2BY, UK
4
Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, USA
5
Department of Materials Science and Engineering, I-Shou University, Kaohsiung 84001, Taiwan
*
Authors to whom correspondence should be addressed.
Biosensors 2020, 10(3), 16; https://doi.org/10.3390/bios10030016
Received: 30 January 2020 / Revised: 21 February 2020 / Accepted: 25 February 2020 / Published: 27 February 2020
Molecularly imprinted polymers (MIPs) can often bind target molecules with high selectivity and specificity. When used as MIPs, conductive polymers may have unique binding capabilities; they often contain aromatic rings and functional groups, which can undergo π-π and hydrogen bonding interactions with similarly structured target (or template) molecules. In this work, an electrochemical method was used to optimize the synthetic self-assembly of poly(aniline-co-metanilic acid) and testosterone, forming testosterone-imprinted electronically conductive polymers (TIECPs) on sensing electrodes. The linear sensing range for testosterone was from 0.1 to 100 pg/mL, and the limit of detection was as low as ~pM. Random urine samples were collected and diluted 1000-fold to measure testosterone concentration using the above TIECP sensors; results were compared with a commercial ARCHITECT ci 8200 system. The testosterone concentrations in the tested samples were in the range of 0.33 ± 0.09 to 9.13 ± 1.33 ng/mL. The mean accuracy of the TIECP-coated sensors was 90.3 ± 7.0%. View Full-Text
Keywords: testosterone; molecular imprinting; electronically conductive polymer; electrochemical sensing; urine testosterone; molecular imprinting; electronically conductive polymer; electrochemical sensing; urine
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Liu, K.-H.; O’Hare, D.; Thomas, J.L.; Guo, H.-Z.; Yang, C.-H.; Lee, M.-H. Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone. Biosensors 2020, 10, 16.

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