Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone

Liu, Kai-Hsi; O’Hare, Danny; Thomas, James L.; Guo, Han-Zhang; Yang, Chien-Hsin; Lee, Mei-Hwa

doi:10.3390/bios10030016

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Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone

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Department of Internal Medicine, Division of Cardiology, Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung 813, Taiwan

Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan

Department of Bioengineering, Imperial College, London SW7 2BY, UK

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Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, USA

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Department of Materials Science and Engineering, I-Shou University, Kaohsiung 84001, Taiwan

Authors to whom correspondence should be addressed.

Biosensors 2020, 10(3), 16; https://doi.org/10.3390/bios10030016

Received: 30 January 2020 / Revised: 21 February 2020 / Accepted: 25 February 2020 / Published: 27 February 2020

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Abstract

Molecularly imprinted polymers (MIPs) can often bind target molecules with high selectivity and specificity. When used as MIPs, conductive polymers may have unique binding capabilities; they often contain aromatic rings and functional groups, which can undergo π-π and hydrogen bonding interactions with similarly structured target (or template) molecules. In this work, an electrochemical method was used to optimize the synthetic self-assembly of poly(aniline-co-metanilic acid) and testosterone, forming testosterone-imprinted electronically conductive polymers (TIECPs) on sensing electrodes. The linear sensing range for testosterone was from 0.1 to 100 pg/mL, and the limit of detection was as low as ~pM. Random urine samples were collected and diluted 1000-fold to measure testosterone concentration using the above TIECP sensors; results were compared with a commercial ARCHITECT ci 8200 system. The testosterone concentrations in the tested samples were in the range of 0.33 ± 0.09 to 9.13 ± 1.33 ng/mL. The mean accuracy of the TIECP-coated sensors was 90.3 ± 7.0%. View Full-Text

Keywords: testosterone; molecular imprinting; electronically conductive polymer; electrochemical sensing; urine

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MDPI and ACS Style

Liu, K.-H.; O’Hare, D.; Thomas, J.L.; Guo, H.-Z.; Yang, C.-H.; Lee, M.-H. Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone. Biosensors 2020, 10, 16. https://doi.org/10.3390/bios10030016

AMA Style

Liu K-H, O’Hare D, Thomas JL, Guo H-Z, Yang C-H, Lee M-H. Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone. Biosensors. 2020; 10(3):16. https://doi.org/10.3390/bios10030016

Chicago/Turabian Style

Liu, Kai-Hsi, Danny O’Hare, James L. Thomas, Han-Zhang Guo, Chien-Hsin Yang, and Mei-Hwa Lee. 2020. "Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone" Biosensors 10, no. 3: 16. https://doi.org/10.3390/bios10030016

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Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone

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