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Open AccessArticle

Detection of the Ovarian Cancer Biomarker Lysophosphatidic Acid in Serum

Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON M5S 3H6, Canada
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Biosensors 2020, 10(2), 13; https://doi.org/10.3390/bios10020013
Received: 23 January 2020 / Revised: 6 February 2020 / Accepted: 11 February 2020 / Published: 14 February 2020
Lysophosphatidic acid (LPA) is present during the medical condition of ovarian cancer at all stages of the disease, and, therefore possesses considerable potential as a biomarker for screening its presence in female patients. Unfortunately, there is currently no clinically employable assay for this biomarker. In the present work, we introduce a test based on the duel protein system of actin and gelsolin that could allow the quantitative measurement of LPA in serum samples in a biosensing format. In order to evaluate this possibility, actin protein was dye-modified and complexed with gelsolin protein, followed by surface deposition onto silica nanoparticles. This solid-phase system was exposed to serum samples containing various concentrations of LPA and analyzed by fluorescence microscopy. Measurements conducted for the LPA-containing serum samples were higher after exposure to the developed test than samples without LPA. Early results suggest a limit of detection of 5 μM LPA in serum. The eventual goal is to employ the chemistry described here in a biosensor configuration for the large population-scale, rapid screening of women for the potential occurrence of ovarian cancer. View Full-Text
Keywords: ovarian cancer; lysophosphatidic acid; fluorescence detection; actin; gelsolin ovarian cancer; lysophosphatidic acid; fluorescence detection; actin; gelsolin
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De La Franier, B.; Thompson, M. Detection of the Ovarian Cancer Biomarker Lysophosphatidic Acid in Serum. Biosensors 2020, 10, 13.

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