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Open AccessArticle

Efficient Production of Multi-Layer Graphene from Graphite Flakes in Water by Lipase-Graphene Sheets Conjugation

Department of Biocatalysis. Institute of Catalysis (CSIC). Marie Curie 2. Cantoblanco. Campus UAM, 28049 Madrid, Spain
Instituto Universitario de Materiales y Departamento de Química Inorgánica, Universidad de Alicante, Apartado 99, San Vicente del Raspeig, E-03080 Alicante, Spain
Author to whom correspondence should be addressed.
Nanomaterials 2019, 9(9), 1344;
Received: 5 September 2019 / Revised: 17 September 2019 / Accepted: 18 September 2019 / Published: 19 September 2019
(This article belongs to the Special Issue Characterization, Synthesis and Applications of 2D Nanomaterials)
Biographene was successfully produced in water from graphite flakes by a simple, rapid, and efficient methodology based on a bioexfoliation technology. The methodology consisted in the application of a lipase, with a unique mechanism of interaction with hydrophobic surfaces, combined with a previous mechanical sonication, to selectively generate lipase-graphene sheets conjugates in water at room temperature. The adsorption of the lipase on the graphene sheets permits to keep the sheets separated in comparison with other methods. It was possible to obtain more than 80% of graphene (in the form of multi-layer graphene) from low-cost graphite and with less damage compared to commercial graphene oxide (GO) or reduced GO. Experimental analysis demonstrated the formation of multi-layer graphene (MLG) mainly using lipase from Thermomyces Lanuginosus (TLL). View Full-Text
Keywords: graphene; exfoliation; lipase; graphite; biographene graphene; exfoliation; lipase; graphite; biographene
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MDPI and ACS Style

Losada-Garcia, N.; Berenguer-Murcia, A.; Cazorla-Amorós, D.; Palomo, J.M. Efficient Production of Multi-Layer Graphene from Graphite Flakes in Water by Lipase-Graphene Sheets Conjugation. Nanomaterials 2019, 9, 1344.

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