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Open AccessArticle

Magnetic-Assisted Cell Alignment within a Magnetic Nanoparticle-Decorated Reduced Graphene Oxide/Collagen 3D Nanocomposite Hydrogel

by 1,†, 2,† and 1,2,*
1
Center for Integrated Biotechnology, Sogang University, 35 Baekbeom-ro (Sinsu-dong), Mapo-gu, Seoul 121-742, Korea
2
Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro (Sinsu-dong), Mapo-gu, Seoul 121-742, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Nanomaterials 2019, 9(9), 1293; https://doi.org/10.3390/nano9091293
Received: 23 July 2019 / Revised: 1 September 2019 / Accepted: 8 September 2019 / Published: 10 September 2019
Hydrogel scaffolds are particularly interesting for applications in tissue engineering because of their ability to create a favorable environment which mimics in vivo conditions. However, the hierarchically ordered anisotropic structure which is found in many native tissues and cellular components is hard to achieve in 3D scaffolds. In this work, we report the incorporation of magnetic nanoparticle-decorated reduced graphene oxide (m-rGO) within a collagen hydrogel. This magneto-responsive m-rGO aligned within the collagen hydrogel during gelation with the application of a low external magnetic field. This nanocomposite hydrogel with magnetically aligned m-rGO flakes is capable of encapsulating neuroblastoma cells (SH-SY5Y), promoting cell differentiation and inducing oriented cell growth owing to its excellent biocompatibility and electrical conductivity. The directionally oriented and differentiated SH-SY5Y cells within the m-rGO collagen hydrogel showed propagation of calcium signal along the direction of orientation. This method can be applied to creating magnetically responsive materials with potential for various biomedical applications. View Full-Text
Keywords: collagen hydrogel; 3D scaffold; reduced graphene oxide; magnetic nanoparticles; aligned cells collagen hydrogel; 3D scaffold; reduced graphene oxide; magnetic nanoparticles; aligned cells
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MDPI and ACS Style

Santhosh, M.; Choi, J.-H.; Choi, J.-W. Magnetic-Assisted Cell Alignment within a Magnetic Nanoparticle-Decorated Reduced Graphene Oxide/Collagen 3D Nanocomposite Hydrogel. Nanomaterials 2019, 9, 1293.

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  • Supplementary File 1:

    PDF-Document (PDF, 1831 KB)

  • Externally hosted supplementary file 1
    Doi: 10.5281/zenodo.3346670
    Link: https://zenodo.org/record/3346670#.XTbWhegzaUk
    Description: Video S1: Calcium image propagation of aligned SH-SY5Y cells within 3D construct Video S2: Calcium image propagation of un-aligned SH-SY5Y cells within 3D construct
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