Nanoencapsulation Enhances Anticoagulant Activity of Adenosine and Dipeptide IleTrp
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Mice
2.3. Preparation of Pluronic P123-grafted Heparin (Hep-P123)
2.4. Preparation of IW and Ado loaded Hep-P123 Nanogels
2.5. Study of IW and Ado Release Behavior
2.6. Determination of Anticoagulant Activity
2.7. Statistical Analysis
3. Results
3.1. Characterization of Hep-P123 Copolymer
3.2. Preparation of IW and Ado Anticoagulants Encapsulated in Hep-P123 Nanogels
3.3. Release Profiles of Ado and IW
3.4. Studies of Anticoagulant Activity
3.4.1. Influence on Blood Coagulation in vitro
3.4.2. Influence on Bleeding Time in vivo
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Cumulative Anticoagulant Release (%) | ||||
---|---|---|---|---|
Time (h) | IW | Ado | Hep-P123-IW | Hep-P123-Ado |
1 | 35.17 ± 1.27 | 36.72 ± 5.54 | 6.48 ± 1.48 | 4.67 ± 2.40 |
3 | 52.08 ± 0.85 | 43.80 ± 4.73 | 12.87 ± 3.01 | 9.03 ± 2.03 |
5 | 59.20 ± 0.70 | 56.53 ± 6.84 | 18.52 ± 1.92 | 18.06 ± 3.61 |
7 | 85.86 ± 1.93 | 75.65 ± 10.10 | 22.95 ± 3.63 | 32.94 ± 4.52 |
9 | 87.13 ± 3.82 | 93.94 ± 10.14 | 28.44 ± 3.62 | 37.81 ± 5.03 |
12 | 93.84 ± 1.05 | 100.0 ± 9.10 | 33.11 ± 4.35 | 40.25 ± 4.04 |
24 | 100.0 ± 4.51 | - | 36.58 ± 7.26 | 45.87 ± 4.71 |
48 | - | - | 43.52 ± 2.64 | 47.59 ± 5.53 |
96 | - | - | 65.94 ± 5.28 | 57.74 ± 4.30 |
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Nguyen, T.D.; Nguyen, T.N.; Nguyen, T.T.T.; Ivanov, I.A.; Nguyen, K.C.; Tran, Q.N.; Hoang, A.N.; Utkin, Y.N. Nanoencapsulation Enhances Anticoagulant Activity of Adenosine and Dipeptide IleTrp. Nanomaterials 2019, 9, 1191. https://doi.org/10.3390/nano9091191
Nguyen TD, Nguyen TN, Nguyen TTT, Ivanov IA, Nguyen KC, Tran QN, Hoang AN, Utkin YN. Nanoencapsulation Enhances Anticoagulant Activity of Adenosine and Dipeptide IleTrp. Nanomaterials. 2019; 9(9):1191. https://doi.org/10.3390/nano9091191
Chicago/Turabian StyleNguyen, Trung Dinh, The Ngoc Nguyen, Trang Thuy Thi Nguyen, Igor A. Ivanov, Khoa Cuu Nguyen, Quyen Ngoc Tran, Anh Ngoc Hoang, and Yuri N. Utkin. 2019. "Nanoencapsulation Enhances Anticoagulant Activity of Adenosine and Dipeptide IleTrp" Nanomaterials 9, no. 9: 1191. https://doi.org/10.3390/nano9091191