Next Article in Journal
Electronic and Optical Properties of Two-Dimensional Tellurene: From First-Principles Calculations
Next Article in Special Issue
Metal Oxide Nanoparticles in Therapeutic Regulation of Macrophage Functions
Previous Article in Journal
Structural Characterization and Digestibility of Curcumin Loaded Octenyl Succinic Nanoparticles
Previous Article in Special Issue
Prediction of Gold Nanoparticle and Microwave-Induced Hyperthermia Effects on Tumor Control via a Simulation Approach
Open AccessArticle

Prolonged Release and Functionality of Interleukin-10 Encapsulated within PLA-PEG Nanoparticles

1
Center for NanoBiotechnology & Life Sciences Research, Department of Biological Sciences, Alabama State University, 915 South Jackson Street, Montgomery, AL 36104, USA
2
New Iberia Research Center, University of Louisiana at Lafayette, 4401 W Admiral Doyle Drive, New Iberia, LA 70560, USA
*
Author to whom correspondence should be addressed.
Nanomaterials 2019, 9(8), 1074; https://doi.org/10.3390/nano9081074
Received: 21 June 2019 / Revised: 9 July 2019 / Accepted: 24 July 2019 / Published: 26 July 2019
(This article belongs to the Special Issue Immune Responses to Nanomaterials for Biomedical Applications)
Inflammation, as induced by the presence of cytokines and chemokines, is an integral part of chlamydial infections. The anti-inflammatory cytokine, interleukin (IL)-10, has been reported to efficiently suppress the secretion of inflammatory cytokines triggered by Chlamydia in mouse macrophages. Though IL-10 is employed in clinical applications, its therapeutic usage is limited due to its short half-life. Here, we document the successful encapsulation of IL-10 within the biodegradable polymeric nanoparticles of PLA-PEG (Poly (lactic acid)-Poly (ethylene glycol), to prolong its half-life. Our results show the encapsulated-IL-10 size (~238 nm), zeta potential (−14.2 mV), polydispersity index (0.256), encapsulation efficiency (~77%), and a prolonged slow release pattern up to 60 days. Temperature stability of encapsulated-IL-10 was favorable, demonstrating a heat capacity of up to 89 °C as shown by differential scanning calorimetry analysis. Encapsulated-IL-10 modulated the release of IL-6 and IL-12p40 in stimulated macrophages in a time- and concentration-dependent fashion, and differentially induced SOCS1 and SOCS3 as induced by chlamydial stimulants in macrophages. Our finding offers the tremendous potential for encapsulated-IL-10 not only for chlamydial inflammatory diseases but also biomedical therapeutic applications. View Full-Text
Keywords: chlamydia; recombinant major outer membrane protein (rMOMP); PLA-PEG nanoparticles; IL-10; cytokines; SOCS1; SOCS3; inflammation; anti-inflammatory; therapy chlamydia; recombinant major outer membrane protein (rMOMP); PLA-PEG nanoparticles; IL-10; cytokines; SOCS1; SOCS3; inflammation; anti-inflammatory; therapy
Show Figures

Figure 1

MDPI and ACS Style

Duncan, S.A.; Dixit, S.; Sahu, R.; Martin, D.; Baganizi, D.R.; Nyairo, E.; Villinger, F.; Singh, S.R.; Dennis, V.A. Prolonged Release and Functionality of Interleukin-10 Encapsulated within PLA-PEG Nanoparticles. Nanomaterials 2019, 9, 1074. https://doi.org/10.3390/nano9081074

AMA Style

Duncan SA, Dixit S, Sahu R, Martin D, Baganizi DR, Nyairo E, Villinger F, Singh SR, Dennis VA. Prolonged Release and Functionality of Interleukin-10 Encapsulated within PLA-PEG Nanoparticles. Nanomaterials. 2019; 9(8):1074. https://doi.org/10.3390/nano9081074

Chicago/Turabian Style

Duncan, Skyla A.; Dixit, Saurabh; Sahu, Rajnish; Martin, David; Baganizi, Dieudonné R.; Nyairo, Elijah; Villinger, Francois; Singh, Shree R.; Dennis, Vida A. 2019. "Prolonged Release and Functionality of Interleukin-10 Encapsulated within PLA-PEG Nanoparticles" Nanomaterials 9, no. 8: 1074. https://doi.org/10.3390/nano9081074

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop