Next Article in Journal
Study on the Physico-Chemical Properties of the Si Nanowires Surface
Next Article in Special Issue
Preparation, Characterization and Adsorption Potential of Grainy Halloysite-CNT Composites for Anthracene Removal from Aqueous Solution
Previous Article in Journal
Technetium Encapsulation by A Nanoporous Complex Oxide 12CaO•7Al2O3 (C12A7)
Previous Article in Special Issue
Preparation of N-Doped Carbon Nanosheets from Sewage Sludge for Adsorption Studies of Cr(VI) from Aqueous Solution
Open AccessArticle

Electrochemical Detection of Ultratrace Lead Ion through Attaching and Detaching DNA Aptamer from Electrochemically Reduced Graphene Oxide Electrode

Department of Chemistry, Soonchunhyang University, Asan 31538, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Nanomaterials 2019, 9(6), 817; https://doi.org/10.3390/nano9060817
Received: 1 May 2019 / Revised: 25 May 2019 / Accepted: 28 May 2019 / Published: 30 May 2019
This paper describes a simple strategy for the ultratrace level detection of Pb2+ ion based on G-quadruplex DNA and an electrochemically reduced graphene oxide (ERGO) electrode. First, ERGO was formed on a glassy carbon electrode (GCE) by the reduction of graphene oxide (GO) using cyclic voltammetry. Subsequently, a methylene blue (MB)-tagged, guanine-rich DNA aptamer (Apt) was attached to the surface of ERGO via π-π interaction, leading to the Apt-modified ERGO electrode. The presence of Pb2+ could generate the folding of Apt to a G-quadruplex structure. The formation of G-quadruplex resulted in detaching the Apt from the ERGO/GCE, leading to a change in redox current of the MB tag. Electrochemical measurements showed the proposed sensor had an exceptional sensitivity for Pb2+ with a linear range from 10−15 to 10−9 M and a detection limit of 0.51 fM. The sensor also exhibited high selectivity for Pb2+, as well as many other advantages, such as stability, reproducibility, regeneration, as well as simple fabrication and operation processes. View Full-Text
Keywords: reduced graphene oxide; G-quadruplex; lead ion; electrochemical aptasensor reduced graphene oxide; G-quadruplex; lead ion; electrochemical aptasensor
Show Figures

Graphical abstract

MDPI and ACS Style

Yu, S.H.; Lee, C.-S.; Kim, T.H. Electrochemical Detection of Ultratrace Lead Ion through Attaching and Detaching DNA Aptamer from Electrochemically Reduced Graphene Oxide Electrode. Nanomaterials 2019, 9, 817.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop