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Open AccessArticle

Development of Effective Lipase-Hybrid Nanoflowers Enriched with Carbon and Magnetic Nanomaterials for Biocatalytic Transformations

1
Biotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, 45110 Ioannina, Greece
2
Department of Materials Science and Engineering, Cornell University, Ithaca, NY 14853, USA
3
Department of Materials Science and Engineering, University of Ioannina, 45110 Ioannina, Greece
*
Author to whom correspondence should be addressed.
Nanomaterials 2019, 9(6), 808; https://doi.org/10.3390/nano9060808
Received: 20 April 2019 / Revised: 19 May 2019 / Accepted: 23 May 2019 / Published: 28 May 2019
(This article belongs to the Special Issue Green Synthesis of Nanomaterials)
In the present study, hybrid nanoflowers (HNFs) based on copper (II) or manganese (II) ions were prepared by a simple method and used as nanosupports for the development of effective nanobiocatalysts through the immobilization of lipase B from Pseudozyma antarctica. The hybrid nanobiocatalysts were characterized by various techniques including scanning electron microscopy (SEM), energy dispersion spectroscopy (EDS), X-ray diffraction (XRD), Raman spectroscopy, and Fourier transform infrared spectroscopy (FTIR). The effect of the addition of carbon-based nanomaterials, namely graphene oxide and carbon nanotubes, as well as magnetic nanoparticles such as maghemite, on the structure, catalytic activity, and operational stability of the hybrid nanobiocatalysts was also investigated. In all cases, the addition of nanomaterials during the preparation of HNFs increased the catalytic activity and the operational stability of the immobilized biocatalyst. Lipase-based magnetic nanoflowers were effectively applied for the synthesis of tyrosol esters in non-aqueous media, such as organic solvents, ionic liquids, and environmental friendly deep eutectic solvents. In such media, the immobilized lipase preserved almost 100% of its initial activity after eight successive catalytic cycles, indicating that these hybrid magnetic nanoflowers can be applied for the development of efficient nanobiocatalytic systems. View Full-Text
Keywords: hybrid nanoflowers; lipase; magnetic nanomaterials; biocatalysis; enzyme immobilization hybrid nanoflowers; lipase; magnetic nanomaterials; biocatalysis; enzyme immobilization
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MDPI and ACS Style

Fotiadou, R.; Patila, M.; Hammami, M.A.; Enotiadis, A.; Moschovas, D.; Tsirka, K.; Spyrou, K.; Giannelis, E.P.; Avgeropoulos, A.; Paipetis, A.; Gournis, D.; Stamatis, H. Development of Effective Lipase-Hybrid Nanoflowers Enriched with Carbon and Magnetic Nanomaterials for Biocatalytic Transformations. Nanomaterials 2019, 9, 808.

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