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Structural Influence on the Post-Clustering Stability of DNA/AgNCs Fluorescence

Department of Systems Biology, College of Life Science and Biotechnology, Institute of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea
UNIK Center for Synthetic Biology, University of Copenhagen, Thorvaldsensvej 40, 2000 Frederiksberg, Copenhagen, Denmark
Author to whom correspondence should be addressed.
Equal Contribution.
Nanomaterials 2019, 9(5), 667;
Received: 6 April 2019 / Accepted: 24 April 2019 / Published: 28 April 2019
(This article belongs to the Special Issue Nanostructured Biosensors)
PDF [2147 KB, uploaded 28 April 2019]


DNA-encapsulated Silver Nanoclusters (DNA/AgNCs) based sensors have gained increasing attention in past years due to their diverse applications in bioimaging, biosensing, and enzymatic assays. Given the potential of DNA/AgNCs for practical applications, the systematic studies of the fluorescent stability over an extended period is necessary. However, the correlation between nucleic acid properties and the long-term stability of DNA/AgNCs is less known. With locking-to-unlocking sensors, in which the secondary structure of DNA template is standardized, we investigated the correlation between the DNA structure and the fluorescence stability of AgNCs. Post-synthesis of DNA/AgNCs, the fluorescence, and structures of templates were monitored over three weeks. By combining the fluorescence spectroscopy with the in-gel fluorescent assay, we found that AgNCs encapsulated by dimer-structured DNA/AgNCs templates were more stable than those of hairpin-structured DNA/AgNCs templates. While the orange fluorescence from the dimer templates increased over three weeks, the red fluorescence from the hairpin templates was diminished by >80% within two days at room temperature. Further tests revealed that hairpin-encapsulated red-emissive AgNCs is more sensitive to oxidation by atmospheric oxygen compared to dimer encapsulated orange AgNCs. Our observations may provide an important clue in encapsulating photophysically more stable AgNCs by tuning the DNA secondary structures. The proposed strategy here can be essential for pragmatic applications of DNA/AgNCs templates. View Full-Text
Keywords: DNA template; silver nanoclusters; nanotechnology; fluorescence; structure; stability DNA template; silver nanoclusters; nanotechnology; fluorescence; structure; stability

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Nagda, R.; Shah, P.; Lee, C.S.; Park, S.; Yang, S.W. Structural Influence on the Post-Clustering Stability of DNA/AgNCs Fluorescence. Nanomaterials 2019, 9, 667.

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