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Nanomaterials 2019, 9(4), 604; https://doi.org/10.3390/nano9040604

Covalent Decoration of Cortical Membranes with Graphene Oxide as a Substrate for Dental Pulp Stem Cells

1
Department of Pharmacy, University “G. d’Annunzio”, Via dei Vestini, 66100 Chieti, Italy
2
Department of Chemical and Pharmaceutical Sciences, University of Trieste, Piazzale Europa 1, 34127 Trieste, Italy
3
Department of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio”, Via dei Vestini, 66100 Chieti, Italy
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 29 March 2019 / Accepted: 8 April 2019 / Published: 12 April 2019
(This article belongs to the Special Issue Carbon Nanomaterials for Therapy, Diagnosis, and Biosensing)
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Abstract

(1) Background: The aim of this study was to optimize, through a cheap and facile protocol, the covalent functionalization of graphene oxide (GO)-decorated cortical membrane (Lamina®) in order to promote the adhesion, the growth and the osteogenic differentiation of DPSCs (Dental Pulp Stem Cells); (2) Methods: GO-coated Laminas were fully characterized by Scannsion Electron Microscopy (SEM) and Atomic Force Microscopy (AFM) analyses. In vitro analyses of viability, membrane integrity and calcium phosphate deposition were performed; (3) Results: The GO-decorated Laminas demonstrated an increase in the roughness of Laminas, a reduction in toxicity and did not affect membrane integrity of DPSCs; and (4) Conclusions: The GO covalent functionalization of Laminas was effective and relatively easy to obtain. The homogeneous GO coating obtained favored the proliferation rate of DPSCs and the deposition of calcium phosphate. View Full-Text
Keywords: graphene oxide; covalent functionalization; cortical membranes; calcium phosphate deposition graphene oxide; covalent functionalization; cortical membranes; calcium phosphate deposition
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Di Carlo, R.; Zara, S.; Ventrella, A.; Siani, G.; Da Ros, T.; Iezzi, G.; Cataldi, A.; Fontana, A. Covalent Decoration of Cortical Membranes with Graphene Oxide as a Substrate for Dental Pulp Stem Cells. Nanomaterials 2019, 9, 604.

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