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Real-Time Monitoring of Biotinylated Molecules Detection Dynamics in Nanoporous Anodic Alumina for Bio-Sensing

Departament d’Enginyeria Electrònica, Elèctrica i Automàtica, ETSE, Universitat Rovira i Virgili, Avda. Països Catalans 26, 43007 Tarragona, Spain
Authors to whom correspondence should be addressed.
Nanomaterials 2019, 9(3), 478;
Received: 22 February 2019 / Revised: 18 March 2019 / Accepted: 19 March 2019 / Published: 23 March 2019
(This article belongs to the Special Issue Technology and Applications of Nanoporous Alumina)
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The chemical modification, or functionalization, of the surfaces of nanomaterials is a key step to achieve biosensors with the best sensitivity and selectivity. The surface modification of biosensors usually comprises several modification steps that have to be optimized. Real-time monitoring of all the reactions taking place during such modification steps can be a highly helpful tool for optimization. In this work, we propose nanoporous anodic alumina (NAA) functionalized with the streptavidin-biotin complex as a platform towards label-free biosensors. Using reflective interferometric spectroscopy (RIfS), the streptavidin-biotin complex formation, using biotinylated thrombin as a molecule model, was monitored in real-time. The study compared the performance of different NAA pore sizes in order to achieve the highest response. Furthermore, the optimal streptavidin concentration that enabled the efficient detection of the biotinylated thrombin attachment was estimated. Finally, the ability of the NAA-RIfS system to quantify the concentration of biotinylated thrombin was evaluated. This study provides an optimized characterization method to monitor the chemical reactions that take place during the biotinylated molecules attachment within the NAA pores. View Full-Text
Keywords: nanoporous anodic alumina; streptavidin; biotin; RIfS; biosensing nanoporous anodic alumina; streptavidin; biotin; RIfS; biosensing

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Pol, L.; Eckstein, C.; Acosta, L.K.; Xifré-Pérez, E.; Ferré-Borrull, J.; Marsal, L.F. Real-Time Monitoring of Biotinylated Molecules Detection Dynamics in Nanoporous Anodic Alumina for Bio-Sensing. Nanomaterials 2019, 9, 478.

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