Assembly and Characterization of HBc Derived Virus-like Particles with Magnetic Core
AbstractCore-virus like particles (VLPs) assembly is a kinetically complex cascade of interactions between viral proteins, nanoparticle’s surface and an ionic environment. Despite many in silico simulations regarding this process, there is still a lack of experimental data. The main goal of this study was to investigate the capsid protein of hepatitis B virus (HBc) assembly into virus-like particles with superparamagnetic iron oxide nanoparticles (SPIONs) as a magnetic core in relation to their characteristics. The native form of HBc was obtained via agroinfection of Nicotiana benthamiana with pEAQ-HBc plasmid. SPIONs of diameter of 15 nm were synthesized and functionalized with two ligands, providing variety in ζ-potential and hydrodynamic diameter. The antigenic potential of the assembled core-VLPs was assessed with enzyme-linked immunosorbent assay (ELISA). Morphology of SPIONs and core-VLPs was evaluated via transmission electron microscopy (TEM). The most successful core-VLPs assembly was obtained for SPIONs functionalized with dihexadecyl phosphate (DHP) at SPIONs/HBc ratio of 0.2/0.05 mg/mL. ELISA results indicate significant decrease of antigenicity concomitant with core-VLPs assembly. In summary, this study provides an experimental assessment of the crucial parameters guiding SPION-HBc VLPs assembly and evaluates the antigenicity of the obtained structures. View Full-Text
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Rybka, J.D.; Mieloch, A.A.; Plis, A.; Pyrski, M.; Pniewski, T.; Giersig, M. Assembly and Characterization of HBc Derived Virus-like Particles with Magnetic Core. Nanomaterials 2019, 9, 155.
Rybka JD, Mieloch AA, Plis A, Pyrski M, Pniewski T, Giersig M. Assembly and Characterization of HBc Derived Virus-like Particles with Magnetic Core. Nanomaterials. 2019; 9(2):155.Chicago/Turabian Style
Rybka, Jakub D.; Mieloch, Adam A.; Plis, Alicja; Pyrski, Marcin; Pniewski, Tomasz; Giersig, Michael. 2019. "Assembly and Characterization of HBc Derived Virus-like Particles with Magnetic Core." Nanomaterials 9, no. 2: 155.
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