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Nanomaterials 2019, 9(2), 155; https://doi.org/10.3390/nano9020155

Assembly and Characterization of HBc Derived Virus-like Particles with Magnetic Core

1
Center for Advanced Technology, Adam Mickiewicz University in Poznań, Umultowska 89C, 61-614 Poznań, Poland
2
Faculty of Chemistry, Adam Mickiewicz University in Poznań, Umultowska 89B, 61-614 Poznań, Poland
3
Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland
4
Institute of Experimental Physics, Freie Universität Berlin, Arnimallee 14, 14195 Berlin, Germany
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 20 December 2018 / Revised: 18 January 2019 / Accepted: 24 January 2019 / Published: 26 January 2019
(This article belongs to the Special Issue Virus-Based Nanomaterials and Nanostructures)
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Abstract

Core-virus like particles (VLPs) assembly is a kinetically complex cascade of interactions between viral proteins, nanoparticle’s surface and an ionic environment. Despite many in silico simulations regarding this process, there is still a lack of experimental data. The main goal of this study was to investigate the capsid protein of hepatitis B virus (HBc) assembly into virus-like particles with superparamagnetic iron oxide nanoparticles (SPIONs) as a magnetic core in relation to their characteristics. The native form of HBc was obtained via agroinfection of Nicotiana benthamiana with pEAQ-HBc plasmid. SPIONs of diameter of 15 nm were synthesized and functionalized with two ligands, providing variety in ζ-potential and hydrodynamic diameter. The antigenic potential of the assembled core-VLPs was assessed with enzyme-linked immunosorbent assay (ELISA). Morphology of SPIONs and core-VLPs was evaluated via transmission electron microscopy (TEM). The most successful core-VLPs assembly was obtained for SPIONs functionalized with dihexadecyl phosphate (DHP) at SPIONs/HBc ratio of 0.2/0.05 mg/mL. ELISA results indicate significant decrease of antigenicity concomitant with core-VLPs assembly. In summary, this study provides an experimental assessment of the crucial parameters guiding SPION-HBc VLPs assembly and evaluates the antigenicity of the obtained structures. View Full-Text
Keywords: virus-like particles; VLPs; hepatitis B virus capsid protein; HBc; viral self-assembly; magnetic core; HBcAg virus-like particles; VLPs; hepatitis B virus capsid protein; HBc; viral self-assembly; magnetic core; HBcAg
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Rybka, J.D.; Mieloch, A.A.; Plis, A.; Pyrski, M.; Pniewski, T.; Giersig, M. Assembly and Characterization of HBc Derived Virus-like Particles with Magnetic Core. Nanomaterials 2019, 9, 155.

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