Fabrication of Gelatin Methacrylate (GelMA) Scaffolds with Nano- and Micro-Topographical and Morphological Features
by
Ana Agustina Aldana 1
, Laura Malatto 2, Muhammad Atiq Ur Rehman 3,4, Aldo Roberto Boccaccini 3,* and Gustavo Abel Abraham 1
Ana Agustina Aldana 1, Laura Malatto 2, Muhammad Atiq Ur Rehman 3,4, Aldo Roberto Boccaccini 3,* and Gustavo Abel Abraham 1
1
Instituto de Investigaciones en Ciencia y Tecnología de Materiales, INTEMA (UNMdP-CONICET), Av. Juan B. Justo 4302, Mar del Plata B7608FDQ, Buenos Aires, Argentina
2
Instituto Nacional de Tecnología Industrial, Centro de Micro y Nanoelectrónica del Bicentenario (INTI-CMNB), Av. Gral. Paz 5445, San Martin B1650KNA, Buenos Aires, Argentina
3
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, Germany
4
Department of Materials Science and Engineering, Institute of Space Technology Islamabad, 1, Islamabad Highway, Islamabad 44000, Pakistan
*
Author to whom correspondence should be addressed.
Nanomaterials 2019, 9(1), 120; https://doi.org/10.3390/nano9010120
Received: 12 December 2018 / Revised: 6 January 2019 / Accepted: 12 January 2019 / Published: 18 January 2019
(This article belongs to the Special Issue Electrospun Nanofibers for Biomedical Applications)
The design of biomimetic biomaterials for cell culture has become a great tool to study and understand cell behavior, tissue degradation, and lesion. Topographical and morphological features play an important role in modulating cell behavior. In this study, a dual methodology was evaluated to generate novel gelatin methacrylate (GelMA)-based scaffolds with nano and micro topographical and morphological features. First, electrospinning parameters and crosslinking processes were optimized to obtain electrospun nanofibrous scaffolds. GelMA mats were characterized by SEM, FTIR, DSC, TGA, contact angle, and water uptake. Various nanofibrous GelMA mats with defect-free fibers and stability in aqueous media were obtained. Then, micropatterned molds produced by photolithography were used as collectors in the electrospinning process. Thus, biocompatible GelMA nanofibrous scaffolds with micro-patterns that mimic extracellular matrix were obtained successfully by combining two micro/nanofabrication techniques, electrospinning, and micromolding. Taking into account the cell viability results, the methodology used in this study could be considered a valuable tool to develop patterned GelMA based nanofibrous scaffolds for cell culture and tissue engineering.
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Keywords:
biomimetic scaffolds; gelatin; electrospinning; micromolding; biomaterials
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MDPI and ACS Style
Aldana, A.A.; Malatto, L.; Rehman, M.A.U.; Boccaccini, A.R.; Abraham, G.A. Fabrication of Gelatin Methacrylate (GelMA) Scaffolds with Nano- and Micro-Topographical and Morphological Features. Nanomaterials 2019, 9, 120.
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