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Open AccessArticle

Distribution of Paramagnetic Fe2O3/SiO2–Core/Shell Nanoparticles in the Rat Lung Studied by Time-of-Flight Secondary Ion Mass Spectrometry: No Indication for Rapid Lipid Adsorption

1
Tascon GmbH, Mendelstraße 17, 48149 Münster, Germany
2
IBE R&D Institute for Lung Health gGmbH, Mendelstraße 11, 48149 Münster, Germany
3
Department of Chemistry & Biology, University of Siegen, Adolf-Reichwein-Str. 2, 57076 Siegen, Germany
*
Author to whom correspondence should be addressed.
Nanomaterials 2018, 8(8), 571; https://doi.org/10.3390/nano8080571
Received: 10 July 2018 / Revised: 23 July 2018 / Accepted: 24 July 2018 / Published: 26 July 2018
(This article belongs to the Special Issue Magnetic Nanoparticles in Biological Applications)
Amorphous silica nanoparticles comprise a class of widely used industrial nanomaterials, which may elicit acute inflammation in the lung. These materials have a large specific surface to which components of the pulmonary micro-milieu can bind. To conduct appropriate binding studies, paramagnetic Fe2O3/SiO2 core/shell nanoparticles (Fe-Si-NP) may be used as an easy-to-isolate silica surrogate, if several prerequisites are fulfilled. To this end, we investigated the distribution of Fe, Si, protein and phosphatidylcholine (PC) by Time-of-Flight secondary ion mass spectrometry (ToF-SIMS) in cryo-sections from the rat lungs to which Fe-Si-NP had been administered for 30 min. Regions-of-interest were identified and analyzed with incident light and enhanced dark-field microscopy (DFM). Fe-Si-NP particles (primary particle size by electron microscopy: 10–20 nm; aggregate size by tracking analysis: 190 ± 20 nm) and agglomerates thereof were mainly attached to alveolar walls and only marginally internalized by cells such as alveolar macrophages. The localization of Fe-Si-NP by DFM was confirmed by ToF-SIMS signals from both, Fe and Si ions. With respect to an optimized signal-to-noise ratio, Fe+, Si+, CH4N+ and the PC head group (C5H15NO4P+) were the most versatile ions to detect iron, silica, protein, and PC, respectively. Largely congruent Fe+ and Si+ signals demonstrated that the silica coating of Fe-Si-NP remained stable under the conditions of the lung. PC, as a major lipid of the pulmonary surfactant, was colocalized with the protein signal alongside alveolar septa, but was not detected on Fe-Si-NP, suggesting that silica nanoparticles do not adsorb lipids of the lung surfactant under native conditions. The study shows that ToF-SIMS is a valuable technique with adequate spatial resolution to analyze nanoparticles together with organic molecules in the lung. The paramagnetic Fe-Si-NP appear well suited to study the binding of proteins to silica nanomaterials in the lung. View Full-Text
Keywords: ToF-SIMS; dark-field microscopy; lipid adsorption; amorphous silica; core/shell nanoparticles; lung tissue; protein corona formation; nanotoxicology; rat ToF-SIMS; dark-field microscopy; lipid adsorption; amorphous silica; core/shell nanoparticles; lung tissue; protein corona formation; nanotoxicology; rat
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MDPI and ACS Style

Veith, L.; Vennemann, A.; Breitenstein, D.; Engelhard, C.; Hagenhoff, B.; Wiemann, M. Distribution of Paramagnetic Fe2O3/SiO2–Core/Shell Nanoparticles in the Rat Lung Studied by Time-of-Flight Secondary Ion Mass Spectrometry: No Indication for Rapid Lipid Adsorption. Nanomaterials 2018, 8, 571. https://doi.org/10.3390/nano8080571

AMA Style

Veith L, Vennemann A, Breitenstein D, Engelhard C, Hagenhoff B, Wiemann M. Distribution of Paramagnetic Fe2O3/SiO2–Core/Shell Nanoparticles in the Rat Lung Studied by Time-of-Flight Secondary Ion Mass Spectrometry: No Indication for Rapid Lipid Adsorption. Nanomaterials. 2018; 8(8):571. https://doi.org/10.3390/nano8080571

Chicago/Turabian Style

Veith, Lothar; Vennemann, Antje; Breitenstein, Daniel; Engelhard, Carsten; Hagenhoff, Birgit; Wiemann, Martin. 2018. "Distribution of Paramagnetic Fe2O3/SiO2–Core/Shell Nanoparticles in the Rat Lung Studied by Time-of-Flight Secondary Ion Mass Spectrometry: No Indication for Rapid Lipid Adsorption" Nanomaterials 8, no. 8: 571. https://doi.org/10.3390/nano8080571

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