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Open AccessArticle

Diazotization-Coupling Reaction-Based Determination of Tyrosine in Urine Using Ag Nanocubes by Surface-Enhanced Raman Spectroscopy

1
Fujian Key Laboratory of Polymer Materials, College of Chemistry and Materials Science, Fuzhou 350007, Fujian, China
2
Center of Engineering Technology Research for Microalgae Germplasm Improvement of Fujian, Southern Institute of Oceanography, Fujian Normal University, Fuzhou 350117, Fujian, China
3
Fujian Key Laboratory of Innate Immune Biology, Biomedical Research Center of South China, College of Life Science, Fujian Normal University, Fuzhou 350117, Fujian, China
4
Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou 350117, Fujian, China
*
Authors to whom correspondence should be addressed.
Nanomaterials 2018, 8(6), 400; https://doi.org/10.3390/nano8060400
Received: 5 May 2018 / Revised: 29 May 2018 / Accepted: 30 May 2018 / Published: 3 June 2018
(This article belongs to the Special Issue Nanostructured Biosensors)
A novel, simple, and highly sensitive method was developed to detect the concentration of tyrosine-derived azo dye indirectly using silver nanocubes (AgNCs) as a substrate on a super-hydrophobic silver film by surface-enhanced Raman spectroscopy (SERS). Diazotization-coupling reaction occurred between diazonium ions and the phenolic tyrosine, resulting in three new typical peaks in the SERS spectrum of the azo dye that was formed on the AgNCs, indicating strong SERS activity. Subsequently, the limit of detection of this approach was as low as 10−12 M for tyrosine. Moreover, the SERS intensities of the three typical SERS signals of the analyte were linearly correlated with the logarithm of concentration of the Tyrosine. The proposed method shows great potential for tyrosine detection in the urine samples of normal humans. View Full-Text
Keywords: silver nanocubes; diazotization-coupling reaction; Tyrosine; Surface enhanced Raman scattering silver nanocubes; diazotization-coupling reaction; Tyrosine; Surface enhanced Raman scattering
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Lu, Y.; Lu, D.; You, R.; Liu, J.; Huang, L.; Su, J.; Feng, S. Diazotization-Coupling Reaction-Based Determination of Tyrosine in Urine Using Ag Nanocubes by Surface-Enhanced Raman Spectroscopy. Nanomaterials 2018, 8, 400.

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