Biocompatible Materials Based on Self-Assembling Peptides on Ti25Nb10Zr Alloy: Molecular Structure and Organization Investigated by Synchrotron Radiation Induced Techniques
by
Valeria Secchi 1
, Stefano Franchi 1,*,†, Marta Santi 1, Alina Vladescu 2, 
Mariana Braic 2, Tomáš Skála 3, Jaroslava Nováková 3, Monica Dettin 4, Annj Zamuner 4, Giovanna Iucci 1 and Chiara Battocchio 1,*
Valeria Secchi 1, Stefano Franchi 1,*,†, Marta Santi 1, Alina Vladescu 2, Mariana Braic 2, Tomáš Skála 3, Jaroslava Nováková 3, Monica Dettin 4, Annj Zamuner 4, Giovanna Iucci 1 and Chiara Battocchio 1,*
1
Department of Science, Roma Tre University of Rome, Via della Vasca Navale 79, 00146 Rome, Italy
2
National Institute for Optoelectronics, 409 Atomistilor St., 077125 Magurele, Romania
3
Department of Surface and Plasma Science, Faculty of Mathematics and Physics, Charles University, V Holešovičkách 2, 18000 Prague, Czech Republic
4
Department of Industrial Engineering, University of Padua, Via Marzolo, 9, Padua 35131, Italy
*
Authors to whom correspondence should be addressed.
†
Present Address: Elettra-Sincrotrone Trieste S.C.p.A. di interesse nazionale, Strada Statale 14-km 163,5 in AREA Science Park, 34149 Basovizza, Trieste, Italy.
Nanomaterials 2018, 8(3), 148; https://doi.org/10.3390/nano8030148
Received: 31 January 2018 / Revised: 28 February 2018 / Accepted: 5 March 2018 / Published: 7 March 2018
(This article belongs to the Special Issue Advanced Synchrotron Radiation Techniques for Nanostructured Materials)
In this work, we applied advanced Synchrotron Radiation (SR) induced techniques to the study of the chemisorption of the Self Assembling Peptide EAbuK16, i.e., H-Abu-Glu-Abu-Glu-Abu-Lys-Abu-Lys-Abu-Glu-Abu-Glu-Abu-Lys-Abu-Lys-NH2 that is able to spontaneously aggregate in anti-parallel β-sheet conformation, onto annealed Ti25Nb10Zr alloy surfaces. This synthetic amphiphilic oligopeptide is a good candidate to mimic extracellular matrix for bone prosthesis, since its β-sheets stack onto each other in a multilayer oriented nanostructure with internal pores of 5–200 nm size. To prepare the biomimetic material, Ti25Nb10Zr discs were treated with aqueous solutions of EAbuK16 at different pH values. Here we present the results achieved by performing SR-induced X-ray Photoelectron Spectroscopy (SR-XPS), angle-dependent Near Edge X-ray Absorption Fine Structure (NEXAFS) spectroscopy, FESEM and AFM imaging on Ti25Nb10Zr discs after incubation with self-assembling peptide solution at five different pH values, selected deliberately to investigate the best conditions for peptide immobilization.
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Keywords:
synchrotron radiation induced spectroscopies; XPS; NEXAFS; nanostructures; titanium alloy; self-assembling peptides; bioactive materials
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
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MDPI and ACS Style
Secchi, V.; Franchi, S.; Santi, M.; Vladescu, A.; Braic, M.; Skála, T.; Nováková, J.; Dettin, M.; Zamuner, A.; Iucci, G.; Battocchio, C. Biocompatible Materials Based on Self-Assembling Peptides on Ti25Nb10Zr Alloy: Molecular Structure and Organization Investigated by Synchrotron Radiation Induced Techniques. Nanomaterials 2018, 8, 148.
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