Stealth Biocompatible Si-Based Nanoparticles for Biomedical Applications
Abstract
:1. Introduction
2. Results and Discussion
2.1. Physico-Chemical Properties of Pristine and Formulated pSiNPs in Stock Suspensions
2.2. In Vitro Biodegradability Kinetics
2.3. Biocompatibility Studies In Vitro
2.4. Biodistribution and Clearance of the pSiNps Formulation In Vivo
2.5. Biocompatibility Studies In Vivo
3. Materials and Methods
3.1. pSiNPs Synthesis
3.2. pSiNPs Functionalization With PEG
3.3. pSiNPs–PEG Functionalization With Man
3.4. pSiNPs Functionalization With Man
3.5. Characterizations of pSiNPs Initial Suspensions in Ethanol
3.6. Dosing of the Phenyl Squarate Mannose Grafted on the pSiNPs–Man and pSiNPs–PEG–Man Formulations
3.7. Physico-Chemical Stability In Vitro
3.8. In Vitro Cytotoxicity on Cell Line
3.9. Biodistribution in Mice
3.10. TNFα Plasma Levels Measurement
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Nanoparticles | pSiNPs | pSiNPs–PEG | pSiNPs–Man | pSiNPs–PEG–Man |
---|---|---|---|---|
Zeta potential (mV) | −25 ± 1 | 28 ± 2 | −18 ± 1 | 10 ± 0.2 |
Hydrodynamic diameter (nm) vol. % | 200 ± 20 | 315 ± 26 | 488 ± 103 | 530 ± 56 |
Hydrodynamic diameter (nm) num. % | 185 ± 18 | 276 ± 35 | 404 ± 33 | 528 ± 77 |
PDI | 0.16 ± 0.02 | 0.35 ± 0.2 | 0.22 ± 0.1 | 0.68 ± 0.3 |
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Liu, W.; Chaix, A.; Gary-Bobo, M.; Angeletti, B.; Masion, A.; Da Silva, A.; Daurat, M.; Lichon, L.; Garcia, M.; Morère, A.; et al. Stealth Biocompatible Si-Based Nanoparticles for Biomedical Applications. Nanomaterials 2017, 7, 288. https://doi.org/10.3390/nano7100288
Liu W, Chaix A, Gary-Bobo M, Angeletti B, Masion A, Da Silva A, Daurat M, Lichon L, Garcia M, Morère A, et al. Stealth Biocompatible Si-Based Nanoparticles for Biomedical Applications. Nanomaterials. 2017; 7(10):288. https://doi.org/10.3390/nano7100288
Chicago/Turabian StyleLiu, Wei, Arnaud Chaix, Magali Gary-Bobo, Bernard Angeletti, Armand Masion, Afitz Da Silva, Morgane Daurat, Laure Lichon, Marcel Garcia, Alain Morère, and et al. 2017. "Stealth Biocompatible Si-Based Nanoparticles for Biomedical Applications" Nanomaterials 7, no. 10: 288. https://doi.org/10.3390/nano7100288