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Open AccessArticle

Size- and Shape-Dependent Antibacterial Studies of Silver Nanoparticles Synthesized by Wet Chemical Routes

1
Centre of Excellence in Solid State Physics, University of the Punjab, QAC, Lahore 54590, Pakistan
2
Department of Zoology, Lahore College for Women University, Jail Road, Lahore 54000, Pakistan
3
Department of MicroBiology & Molecular Genetics, University of the Punjab, QAC, Lahore 54590, Pakistan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Thomas Nann
Nanomaterials 2016, 6(4), 74; https://doi.org/10.3390/nano6040074
Received: 5 January 2016 / Revised: 19 February 2016 / Accepted: 2 March 2016 / Published: 15 April 2016
(This article belongs to the Special Issue Recent Advances in Nanomaterials’ Research: Selection from ICSSP'15)
Silver nanoparticles (AgNPs) of different shapes and sizes were prepared by solution-based chemical reduction routes. Silver nitrate was used as a precursor, tri-sodium citrate (TSC) and sodium borohydride as reducing agents, while polyvinylpyrrolidone (PVP) was used as a stabilizing agent. The morphology, size, and structural properties of obtained nanoparticles were characterized by scanning electron microscopy (SEM), UV-visible spectroscopy (UV-VIS), and X-ray diffraction (XRD) techniques. Spherical AgNPs, as depicted by SEM, were found to have diameters in the range of 15 to 90 nm while lengths of the edges of the triangular particles were about 150 nm. The characteristic surface plasmon resonance (SPR) peaks of different spherical silver colloids occurring in the wavelength range of 397 to 504 nm, whereas triangular particles showed two peaks, first at 392 nm and second at 789 nm as measured by UV-VIS. The XRD spectra of the prepared samples indicated the face-centered cubic crystalline structure of metallic AgNPs. The in vitro antibacterial properties of all synthesized AgNPs against two types of Gram-negative bacteria, Pseudomonas aeruginosa and Escherichia coli were examined by Kirby–Bauer disk diffusion susceptibility method. It was noticed that the smallest-sized spherical AgNPs demonstrated a better antibacterial activity against both bacterial strains as compared to the triangular and larger spherical shaped AgNPs. View Full-Text
Keywords: AgNPs; reduction method; antibacterial activity; Pseudomonas aeruginosa (P. aeruginosa); Escherichia coli (E. coli) AgNPs; reduction method; antibacterial activity; Pseudomonas aeruginosa (P. aeruginosa); Escherichia coli (E. coli)
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MDPI and ACS Style

Raza, M.A.; Kanwal, Z.; Rauf, A.; Sabri, A.N.; Riaz, S.; Naseem, S. Size- and Shape-Dependent Antibacterial Studies of Silver Nanoparticles Synthesized by Wet Chemical Routes. Nanomaterials 2016, 6, 74.

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