Construction of Double-Shelled Hollow Ag2S@Polydopamine Nanocomposites for Fluorescence-Guided, Dual Stimuli-Responsive Drug Delivery and Photothermal Therapy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Characterization
2.3. Preparation of Mesoporous Silica-Coated Hollow Ag2S NPs (HAg2S@mSiO2)
2.4. Preparation of MPDA-Coated HAg2S@mSiO2 Nanoparticles (HAg2S@mSiO2@MPDA)
2.5. Preparation of HAg2S@HMPDA/PEI Nanoparticles
2.6. Preparation of HAg2S@HMPDA/LA Nanoparticles
2.7. Loading of DOX on HAg2S@HMPDA/LA (HAg2S@HMPDA/LA-DOX)
2.8. Photothermal Performance of HAg2S@HMPDA/LA
2.9. In Vitro pH-Triggered Release of DOX from HAg2S@HMPDA/LA-DOX
2.10. In Vitro NIR-Triggered Release of DOX from HAg2S@HMPDA/LA-DOX
2.11. In Vitro Cytotoxicity Assay and Photothermal–Chemotherapy
2.12. In Vitro Targeting Ability of HAg2S@HMPDA/LA
2.13. In Vitro Imaging of HAg2S@HMPDA/LA
3. Results
3.1. Characterization of HAg2S@HMPDA/LA
3.2. Photothermal Property of HAg2S@HMPDA/LA
3.3. pH/NIR-Responsive Drug Release Activities
3.4. In Vitro Cytotoxicity Assay and Synergistic Photothermal–Chemotherapy
3.5. In Vitro Cancer Cell Targeting Effect
3.6. Cellular Uptake and Imaging
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Gao, M.; Han, Z.; Zhang, X.; Zou, X.; Peng, L.; Zhao, Y.; Sun, L. Construction of Double-Shelled Hollow Ag2S@Polydopamine Nanocomposites for Fluorescence-Guided, Dual Stimuli-Responsive Drug Delivery and Photothermal Therapy. Nanomaterials 2022, 12, 2068. https://doi.org/10.3390/nano12122068
Gao M, Han Z, Zhang X, Zou X, Peng L, Zhao Y, Sun L. Construction of Double-Shelled Hollow Ag2S@Polydopamine Nanocomposites for Fluorescence-Guided, Dual Stimuli-Responsive Drug Delivery and Photothermal Therapy. Nanomaterials. 2022; 12(12):2068. https://doi.org/10.3390/nano12122068
Chicago/Turabian StyleGao, Minjie, Zehua Han, Xu Zhang, Xueyan Zou, Lichao Peng, Yanbao Zhao, and Lei Sun. 2022. "Construction of Double-Shelled Hollow Ag2S@Polydopamine Nanocomposites for Fluorescence-Guided, Dual Stimuli-Responsive Drug Delivery and Photothermal Therapy" Nanomaterials 12, no. 12: 2068. https://doi.org/10.3390/nano12122068