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Article

Comparison of Duplex and Quadruplex Folding Structure Adenosine Aptamers for Carbon Nanotube Field Effect Transistor Aptasensors

1
School of Chemical and Physical Sciences, Victoria University of Wellington, Wellington 6021, New Zealand
2
The MacDiarmid Institute for Advanced Materials and Nanotechnolgy, Victoria University of Wellington, Wellington 6021, New Zealand
3
Department of Physics, University of Jaffna, Jaffna 40000, Sri Lanka
*
Author to whom correspondence should be addressed.
Academic Editor: Mireia Baeza
Nanomaterials 2021, 11(9), 2280; https://doi.org/10.3390/nano11092280
Received: 30 July 2021 / Revised: 25 August 2021 / Accepted: 30 August 2021 / Published: 2 September 2021
(This article belongs to the Special Issue Carbon-Based Nanocomposites for Biosensing Approaches)
Carbon nanotube field effect transistor (CNT FET) aptasensors have been investigated for the detection of adenosine using two different aptamer sequences, a 35-mer and a 27-mer. We found limits of detection for adenosine of 100 pM and 320 nM for the 35-mer and 27-mer aptamers, with dissociation constants of 1.2 nM and 160 nM, respectively. Upon analyte recognition the 35-mer adenosine aptamer adopts a compact G-quadruplex structure while the 27-mer adenosine aptamer changes to a folded duplex. Using the CNT FET aptasensor platform adenosine could be detected with high sensitivity over the range of 100 pM to 10 µM, highlighting the suitability of the CNT FET aptasensor platform for high performance adenosine detection. The aptamer restructuring format is critical for high sensitivity with the G-quadraplex aptasensor having a 130-fold smaller dissociation constant than the duplex forming aptasensor. View Full-Text
Keywords: aptasensor; carbon nanotube FET; adenosine; adenosine detection; aptamer; biosensor aptasensor; carbon nanotube FET; adenosine; adenosine detection; aptamer; biosensor
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MDPI and ACS Style

Nguyen, H.P.T.; Murugathas, T.; Plank, N.O.V. Comparison of Duplex and Quadruplex Folding Structure Adenosine Aptamers for Carbon Nanotube Field Effect Transistor Aptasensors. Nanomaterials 2021, 11, 2280. https://doi.org/10.3390/nano11092280

AMA Style

Nguyen HPT, Murugathas T, Plank NOV. Comparison of Duplex and Quadruplex Folding Structure Adenosine Aptamers for Carbon Nanotube Field Effect Transistor Aptasensors. Nanomaterials. 2021; 11(9):2280. https://doi.org/10.3390/nano11092280

Chicago/Turabian Style

Nguyen, Hong Phan T., Thanihaichelvan Murugathas, and Natalie O. V. Plank. 2021. "Comparison of Duplex and Quadruplex Folding Structure Adenosine Aptamers for Carbon Nanotube Field Effect Transistor Aptasensors" Nanomaterials 11, no. 9: 2280. https://doi.org/10.3390/nano11092280

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