In Vitro Effect of Replicated Porous Polymeric Nano-MicroStructured Biointerfaces Characteristics on Macrophages Behavior
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Reagents
2.2. Method
2.2.1. Pyramid-Shaped Micropatterns PDMS Mold
2.2.2. Porous Microstructured PVDF Preparation
2.3. Microstructured PVDF Replica Topography Analysis by SEM and AFM
2.3.1. Scanning Electron Microscopy
2.3.2. Atomic Force Microscopy
2.4. Wettability and Surface Free Energy Characterization
2.4.1. Contact Angle (CA)
2.4.2. Surface Free Energy Measurements (SFE)
2.5. Replica PVDF–Chemical Profile: FTIR and XPS Analysis
2.5.1. Fourier-Transform Infrared Spectroscopy (FT-IR) Analysis
2.5.2. X-ray Photoelectron Spectroscopy
2.6. Biological Investigations of PVDF Replicated Surfaces
2.6.1. Sterilization Procedure
2.6.2. Cell Culture
2.6.3. In Vitro Macrophage Stimulation Assay
2.6.4. Cell Viability
2.6.5. Cell Adhesion and Morphology
2.6.6. Cytokine Secretion
2.6.7. Statistical Analysis
3. Results and Discussions
3.1. PVDF-Replica Morphology Analysis
3.2. Chemical Characterization of the Replicated PVDF
3.3. Wettability
3.4. Biological In Vitro Investigations
3.4.1. Cell Viability
3.4.2. Cell Adhesion and Morphology
3.4.3. Cytokine Release
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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β-Phase | Replicated PVDF |
---|---|
839 cm−1 | related to C–C–C asymmetrical stretching vibration and CF stretching vibration (deformation of CF2) |
1071 and 1178 cm−1 | symmetrical stretching of CF2 group |
γ-Phase | Replicated PVDF |
1233 cm−1 | γ- polymorph phase |
PVDF | Atomic Ratio | |
---|---|---|
F/C | O/C | |
Pristine | 0.86 | 0.08 |
Replicated | 1.36 | 0.46 |
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Dumitrescu, L.N.; Icriverzi, M.; Bonciu, A.; Roșeanu, A.; Moldovan, A.; Dinca, V. In Vitro Effect of Replicated Porous Polymeric Nano-MicroStructured Biointerfaces Characteristics on Macrophages Behavior. Nanomaterials 2021, 11, 1913. https://doi.org/10.3390/nano11081913
Dumitrescu LN, Icriverzi M, Bonciu A, Roșeanu A, Moldovan A, Dinca V. In Vitro Effect of Replicated Porous Polymeric Nano-MicroStructured Biointerfaces Characteristics on Macrophages Behavior. Nanomaterials. 2021; 11(8):1913. https://doi.org/10.3390/nano11081913
Chicago/Turabian StyleDumitrescu, Luminita Nicoleta, Madalina Icriverzi, Anca Bonciu, Anca Roșeanu, Antoniu Moldovan, and Valentina Dinca. 2021. "In Vitro Effect of Replicated Porous Polymeric Nano-MicroStructured Biointerfaces Characteristics on Macrophages Behavior" Nanomaterials 11, no. 8: 1913. https://doi.org/10.3390/nano11081913