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Article

Gold-Conjugated Nanobodies for Targeted Imaging Using High-Resolution Secondary Ion Mass Spectrometry

1
Center for Biostructural Imaging of Neurodegeneration, University Medical Center Göttingen, von-Siebold-Straße 3a, 37075 Göttingen, Germany
2
Department of Neuro- and Sensory Physiology, University Medical Center Göttingen, Humboldtallee 23, 37073 Göttingen, Germany
3
Department of Chemistry and Molecular Biology, University of Gothenburg, Kemivägen 10, 41296 Gothenburg, Sweden
*
Author to whom correspondence should be addressed.
Academic Editor: Pasquina Marzola
Nanomaterials 2021, 11(7), 1797; https://doi.org/10.3390/nano11071797
Received: 27 May 2021 / Revised: 7 July 2021 / Accepted: 8 July 2021 / Published: 10 July 2021
(This article belongs to the Special Issue Nanomaterials for Contrast Agent and Biomedical Imaging)
Nanoscale imaging with the ability to identify cellular organelles and protein complexes has been a highly challenging subject in the secondary ion mass spectrometry (SIMS) of biological samples. This is because only a few isotopic tags can be used successfully to target specific proteins or organelles. To address this, we generated gold nanoprobes, in which gold nanoparticles are conjugated to nanobodies. The nanoprobes were well suited for specific molecular imaging using NanoSIMS at subcellular resolution. They were demonstrated to be highly selective to different proteins of interest and sufficiently sensitive for SIMS detection. The nanoprobes offer the possibility of correlating the investigation of cellular isotopic turnover to the positions of specific proteins and organelles, thereby enabling an understanding of functional and structural relations that are currently obscure. View Full-Text
Keywords: nanobodies; gold nanoparticles; target imaging; NanoSIMS; SIMS nanobodies; gold nanoparticles; target imaging; NanoSIMS; SIMS
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MDPI and ACS Style

Agüi-Gonzalez, P.; Dankovich, T.M.; Rizzoli, S.O.; Phan, N.T.N. Gold-Conjugated Nanobodies for Targeted Imaging Using High-Resolution Secondary Ion Mass Spectrometry. Nanomaterials 2021, 11, 1797. https://doi.org/10.3390/nano11071797

AMA Style

Agüi-Gonzalez P, Dankovich TM, Rizzoli SO, Phan NTN. Gold-Conjugated Nanobodies for Targeted Imaging Using High-Resolution Secondary Ion Mass Spectrometry. Nanomaterials. 2021; 11(7):1797. https://doi.org/10.3390/nano11071797

Chicago/Turabian Style

Agüi-Gonzalez, Paola, Tal M. Dankovich, Silvio O. Rizzoli, and Nhu T. N. Phan. 2021. "Gold-Conjugated Nanobodies for Targeted Imaging Using High-Resolution Secondary Ion Mass Spectrometry" Nanomaterials 11, no. 7: 1797. https://doi.org/10.3390/nano11071797

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