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Article

Dielectric Imaging of Fixed HeLa Cells by In-Liquid Scanning Dielectric Force Volume Microscopy

1
Nanoscale Bioelectric Characterization, Institut de Bioenginyeria de Catalunya (IBEC), The Barcelona Institute of Science and Technology (BIST), c/Baldiri I Reixac 11-15, 08028 Barcelona, Spain
2
Departament d’Enginyeria Electrònica i Biomèdica, Universitat de Barcelona, c/Martí i Franquès 1, 08028 Barcelona, Spain
3
Nanoscopy for Nanomedicine, Institut de Bioenginyeria de Catalunya (IBEC), The Barcelona Institute of Science and Technology (BIST), c/Baldiri I Reixac 11-15, 08028 Barcelona, Spain
*
Authors to whom correspondence should be addressed.
Present address: ICN2—Institut Català de Nanociència I Nanotecnologia (CERCA-BIST-CSIC), Campus Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.
Academic Editor: Stefano Leporatti
Nanomaterials 2021, 11(6), 1402; https://doi.org/10.3390/nano11061402
Received: 10 May 2021 / Revised: 21 May 2021 / Accepted: 23 May 2021 / Published: 25 May 2021
(This article belongs to the Special Issue Microscopy for Nanomedicine Research)
Mapping the dielectric properties of cells with nanoscale spatial resolution can be an important tool in nanomedicine and nanotoxicity analysis, which can complement structural and mechanical nanoscale measurements. Recently we have shown that dielectric constant maps can be obtained on dried fixed cells in air environment by means of scanning dielectric force volume microscopy. Here, we demonstrate that such measurements can also be performed in the much more challenging case of fixed cells in liquid environment. Performing the measurements in liquid media contributes to preserve better the structure of the fixed cells, while also enabling accessing the local dielectric properties under fully hydrated conditions. The results shown in this work pave the way to address the nanoscale dielectric imaging of living cells, for which still further developments are required, as discussed here. View Full-Text
Keywords: scanning probe microscopy (SPM); electrostatic force microscopy (EFM); scanning dielectric microscopy (SDM); atomic force microscopy (AFM); dielectric properties; functional microscopy scanning probe microscopy (SPM); electrostatic force microscopy (EFM); scanning dielectric microscopy (SDM); atomic force microscopy (AFM); dielectric properties; functional microscopy
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MDPI and ACS Style

Checa, M.; Millan-Solsona, R.; Glinkowska Mares, A.; Pujals, S.; Gomila, G. Dielectric Imaging of Fixed HeLa Cells by In-Liquid Scanning Dielectric Force Volume Microscopy. Nanomaterials 2021, 11, 1402. https://doi.org/10.3390/nano11061402

AMA Style

Checa M, Millan-Solsona R, Glinkowska Mares A, Pujals S, Gomila G. Dielectric Imaging of Fixed HeLa Cells by In-Liquid Scanning Dielectric Force Volume Microscopy. Nanomaterials. 2021; 11(6):1402. https://doi.org/10.3390/nano11061402

Chicago/Turabian Style

Checa, Martí, Ruben Millan-Solsona, Adrianna Glinkowska Mares, Silvia Pujals, and Gabriel Gomila. 2021. "Dielectric Imaging of Fixed HeLa Cells by In-Liquid Scanning Dielectric Force Volume Microscopy" Nanomaterials 11, no. 6: 1402. https://doi.org/10.3390/nano11061402

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