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Article

Comparative Study of Gold and Carbon Nanoparticles in Nucleic Acid Lateral Flow Assay

1
Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
2
Institute of Biotechnology and Biomedicine, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
*
Author to whom correspondence should be addressed.
Academic Editors: Antonino Mazzaglia and Elena Aznar
Nanomaterials 2021, 11(3), 741; https://doi.org/10.3390/nano11030741
Received: 15 January 2021 / Revised: 1 March 2021 / Accepted: 8 March 2021 / Published: 15 March 2021
(This article belongs to the Special Issue Application of Carbon Nanomaterials in Biological Detection)
A lateral flow assay (LFA) is a paper-based, point-of-need test designed to detect a specific analyte in complex samples in low-resource settings. Although LFA has been successfully used in different applications, its use is still limited when high sensitivity is required, especially in the diagnosis of an early-stage condition. The limit of detection (LOD) is clearly related to the signal-generating system used to achieve the visual readout, in many cases involving nanoparticles coupled to a biomolecule, which, when combined, provides sensitivity and specificity, respectively. While colloidal gold is currently the most-used label, other detection systems are being developed. Carbon nanoparticles (CNPs) demonstrate outstanding features to improve the sensitivity of this technology by producing an increased contrast in the paper background. Based on the necessity of sensitivity improvement, the aim of this work is a comparative study, in terms of analytical performance, between commercial streptavidin gold nanoparticles (streptAv-AuNPs) and avidin carbon nanoparticles (Av-CNPs) in a nucleic acid lateral flow assay. The visual LOD of the method was calculated by serial dilution of the DNA template, ranging from 0.0 to 7 pg μL−1/1.5 × 104 CFU mL−1). The LFA achieved visual detection of as low as 2.2 × 10−2 pg μL−1 using Av-CNPs and 8.4 × 10−2 pg μL−1 using streptAv-AuNPs. These LODs could be obtained without the assistance of any instrumentation. The results demonstrate that CNPs showed an increased sensitivity, achieving the nanomolar range even by visual inspection. Furthermore, CNPs are the cheapest labels, and the suspensions are very stable and easy to modify. View Full-Text
Keywords: carbon black; gold nanoparticles; rapid diagnostic tests; lateral flow assay carbon black; gold nanoparticles; rapid diagnostic tests; lateral flow assay
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MDPI and ACS Style

Porras, J.C.; Bernuz, M.; Marfa, J.; Pallares-Rusiñol, A.; Martí, M.; Pividori, M.I. Comparative Study of Gold and Carbon Nanoparticles in Nucleic Acid Lateral Flow Assay. Nanomaterials 2021, 11, 741. https://doi.org/10.3390/nano11030741

AMA Style

Porras JC, Bernuz M, Marfa J, Pallares-Rusiñol A, Martí M, Pividori MI. Comparative Study of Gold and Carbon Nanoparticles in Nucleic Acid Lateral Flow Assay. Nanomaterials. 2021; 11(3):741. https://doi.org/10.3390/nano11030741

Chicago/Turabian Style

Porras, Juan C., Mireia Bernuz, Jennifer Marfa, Arnau Pallares-Rusiñol, Mercè Martí, and María I. Pividori 2021. "Comparative Study of Gold and Carbon Nanoparticles in Nucleic Acid Lateral Flow Assay" Nanomaterials 11, no. 3: 741. https://doi.org/10.3390/nano11030741

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