New Insights into the Cell Death Signaling Pathways Triggered by Long-Term Exposure to Silicon-Based Quantum Dots in Human Lung Fibroblasts
Abstract
:1. Introduction
2. Experimental Section
2.1. QDs Synthesis and Characterization
2.2. Cell Culture and Long-Term Exposure to QDs
2.3. Trypan Blue Staining
2.4. TEM Analysis of Cell Samples
2.5. Western Blot
2.6. Southern Blot
2.7. Statistical Analysis
3. Results and Discussion
3.1. Characterization of Si/SiO2 QDs
3.2. Analysis of MRC-5 Cells’ Growth during the Long-Term Exposure to QDs
3.3. QDs Internalization into MRC-5 Cells during the Long-Term Exposure
3.4. Effects of Long-Term Exposure to QDs of Autophagy Pathway in MRC-5 Cells
3.5. Effects of Long-Term Exposure to QDs of Apoptosis Pathways in MRC-5 cells
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Stan, M.S.; Badea, S.; Hermenean, A.; Herman, H.; Trica, B.; Sbarcea, B.G.; Dinischiotu, A. New Insights into the Cell Death Signaling Pathways Triggered by Long-Term Exposure to Silicon-Based Quantum Dots in Human Lung Fibroblasts. Nanomaterials 2021, 11, 323. https://doi.org/10.3390/nano11020323
Stan MS, Badea S, Hermenean A, Herman H, Trica B, Sbarcea BG, Dinischiotu A. New Insights into the Cell Death Signaling Pathways Triggered by Long-Term Exposure to Silicon-Based Quantum Dots in Human Lung Fibroblasts. Nanomaterials. 2021; 11(2):323. https://doi.org/10.3390/nano11020323
Chicago/Turabian StyleStan, Miruna S., Smaranda Badea, Anca Hermenean, Hildegard Herman, Bogdan Trica, Beatrice G. Sbarcea, and Anca Dinischiotu. 2021. "New Insights into the Cell Death Signaling Pathways Triggered by Long-Term Exposure to Silicon-Based Quantum Dots in Human Lung Fibroblasts" Nanomaterials 11, no. 2: 323. https://doi.org/10.3390/nano11020323