Simultaneous Ultrasound-Assisted Hybrid Polyzwitterion/Antimicrobial Peptide Nanoparticles Synthesis and Deposition on Silicone Urinary Catheters for Prevention of Biofilm-Associated Infections
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Ultrasound Coating of Silicone Materials with Polymyxin B and pSBMA NPs
2.3. Surface-Morphology and Surface-Wetting Characterization
2.4. Protein Adsorption Test
2.5. Antibacterial Activity of pSBMA@PM Coated Catheters
2.6. Biofilm Inhibition Properties
2.6.1. Crystal Violet Assay
2.6.2. Cell Viability Assay
2.6.3. Live/Dead Kit Assay
2.7. Dynamic Biofilm Inhibition Tests
2.8. Biocompatibility Assessment
3. Results and Discussion
3.1. Silicone Functionalization with Hybrid pSBMA@PM NPs
3.2. Initial Protein Attachment
3.3. Antibacterial Activity of the Hybrid pSBMA@PM NP Coating
3.4. Inhibition of P. aeruginosa Biofilm Formation by the Hybrid pSBMA@PM NP Coating
3.5. Biofilm Inhibition Tests in an In Vitro Model of Catheterized Bladder
3.6. Biocompatibility Assessments
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Ivanova, A.; Ivanova, K.; Tzanov, T. Simultaneous Ultrasound-Assisted Hybrid Polyzwitterion/Antimicrobial Peptide Nanoparticles Synthesis and Deposition on Silicone Urinary Catheters for Prevention of Biofilm-Associated Infections. Nanomaterials 2021, 11, 3143. https://doi.org/10.3390/nano11113143
Ivanova A, Ivanova K, Tzanov T. Simultaneous Ultrasound-Assisted Hybrid Polyzwitterion/Antimicrobial Peptide Nanoparticles Synthesis and Deposition on Silicone Urinary Catheters for Prevention of Biofilm-Associated Infections. Nanomaterials. 2021; 11(11):3143. https://doi.org/10.3390/nano11113143
Chicago/Turabian StyleIvanova, Aleksandra, Kristina Ivanova, and Tzanko Tzanov. 2021. "Simultaneous Ultrasound-Assisted Hybrid Polyzwitterion/Antimicrobial Peptide Nanoparticles Synthesis and Deposition on Silicone Urinary Catheters for Prevention of Biofilm-Associated Infections" Nanomaterials 11, no. 11: 3143. https://doi.org/10.3390/nano11113143