Photoactivable Ruthenium-Based Coordination Polymer Nanoparticles for Light-Induced Chemotherapy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents and Instrumentation
2.2. Synthesis and Characterization of the Photoactive Materials
2.2.1. Synthesis of Complex 2
2.2.2. Synthesis of Coordination Polymer Nanoparticles RuBIS
2.3. HPLC Methodology for RuBIS CPNs Releasing Quantification
2.4. Quantitative 1H NMR and 19F NMR for Component Analysis of RuBIS CPNs
2.5. In Vitro Studies
2.5.1. Cell Culturing
2.5.2. Cell-Irradiation Setup
2.5.3. Cytotoxicity Assay
2.5.4. Cellular Uptake Measurements
2.5.5. Endocytosis Inhibition Studies
2.5.6. ICP-MS Analysis
2.5.7. Singlet Oxygen (1O2) Production Studies
3. Results and Discussion
3.1. Synthesis and Characterization
3.2. Photoreactivity of RuBIS CPNs
3.2.1. Monitorization by UV-Vis
3.2.2. HPLC Studies
3.3. Cellular Uptake Measurements
3.4. (Photo)cytotoxicity Studies
3.5. Singlet Oxygen Production
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cell Type | Light DoseJ/cm2 | RuBIS CPNs | Complex 2 | Cisplatin | ||||||
---|---|---|---|---|---|---|---|---|---|---|
EC50 (µM) | CI [a] | PI [b] | EC50(µM) | CI [a] | PI [b] | EC50(µM) | CI [a] | PI [b] | ||
A431 | 0 | 11.9 | +0.46 −n.a. | 2.4 | 28.1 | +0.06 −0.60 | 1.7 | 3.0 | +0.45 −0.41 | 1.1 |
39.3 | 5.0 | +0.04 −0.04 | 16.3 | +0.55 −0.32 | 3.3 | +0.31 −0.28 | ||||
A549 | 0 | 9.1 | +0.09 −0.08 | 1.8 | 28.3 | +1.16 −0.74 | 1.0 | 3.0 | +0.15 −0.15 | 1.0 |
39.3 | 5.0 | +0.02 −0.02 | 27.5 | +0.43 −0.37 | 3.0 | +0.17 −0.17 |
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Zhang, J.; Ramu, V.; Zhou, X.-Q.; Frias, C.; Ruiz-Molina, D.; Bonnet, S.; Roscini, C.; Novio, F. Photoactivable Ruthenium-Based Coordination Polymer Nanoparticles for Light-Induced Chemotherapy. Nanomaterials 2021, 11, 3089. https://doi.org/10.3390/nano11113089
Zhang J, Ramu V, Zhou X-Q, Frias C, Ruiz-Molina D, Bonnet S, Roscini C, Novio F. Photoactivable Ruthenium-Based Coordination Polymer Nanoparticles for Light-Induced Chemotherapy. Nanomaterials. 2021; 11(11):3089. https://doi.org/10.3390/nano11113089
Chicago/Turabian StyleZhang, Junda, Vadde Ramu, Xue-Quan Zhou, Carolina Frias, Daniel Ruiz-Molina, Sylvestre Bonnet, Claudio Roscini, and Fernando Novio. 2021. "Photoactivable Ruthenium-Based Coordination Polymer Nanoparticles for Light-Induced Chemotherapy" Nanomaterials 11, no. 11: 3089. https://doi.org/10.3390/nano11113089