Co-Loading of Temozolomide and Curcumin into a Calix[4]arene-Based Nanocontainer for Potential Combined Chemotherapy: Binding Features, Enhanced Drug Solubility and Stability in Aqueous Medium
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Instrumentation
2.3. Synthesis and Characterization of SC4OC6
2.4. Preparation and Characterization of the Micellar SC4OC6 Nanoparticles
2.5. ITC Titrations
2.6. Loading of TMZ in Micellar SC4OC6 Nanoparticles
2.7. Loading of CUR in Micellar SC4OC6 Nanoparticles
2.8. Loading of TMZ in SC4OC6 /CUR Nanoassembly
2.9. Determination of Loaded TMZ and CUR
2.10. CUR Loading Capacity% (LC%)
2.11. TMZ and CUR Stability
3. Results and Discussion
3.1. Preparation and Characterization of the Micellar SC4OC6 Nanoassembly
3.2. Preparation and Characterization of the SC4OC6/CUR Nanoassembly
3.2.1. Solubility of CUR in the Presence of Micellar SC4OC6 Nanoparticle
3.2.2. Stability of CUR in the SC4OC6 Nanoassembly
3.2.3. Dimensional Analysis and Zeta Potential of the SC4OC6/CUR Nanoassembly
3.3. Preparation and Characterization of the SC4OC6/TMZ Nanoassembly
3.3.1. Solubility of TMZ in the Presence of the Micellar SC4OC6 Nanoparticle
3.3.2. Characterization of SC4OC6/TMZ Inclusion Complex
3.3.3. Dimensional Analysis and Zeta Potential of the SC4OC6/TMZ Complex
3.3.4. Stability of TMZ when Complexed with SC4OC6
3.4. Co-Entrapment of TMZ and CUR in the SC4OC6 Nanocontainer
Dimensional and Zeta Potential Analysis of SC4OC6/CUR/TMZ
3.5. Solution Thermodynamics of the SC4OC6/TMZ and SC4OC6/CUR/TMZ Complexes Formation
3.6. Stability of TMZ in the Ternary SC4OC6/CUR/TMZ Assembly
3.7. Potential of SC4OC6/CUR/TMZ in Nanocarrier-Based Combined Chemotherapy
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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log K | ΔH (kJ mol−1) | ΔS (J K−1 mol−1) | |
---|---|---|---|
(TMZ)(SC4OC6)below CMC | n.d. | n.d. | n.d. |
(TMZ)(SC4OC6)above CMC | 2.8 (1) | −5.75 (6) | 34 (2) |
(TMZ)(SC4OC6-CUR)above CMC | 2.9 (1) | −5.62 (4) | 36 (2) |
(TMZ)(SC4OH) | 2.2 (1) | −15.82 (7) | −25 (2) |
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Migliore, R.; D’Antona, N.; Sgarlata, C.; Consoli, G.M.L. Co-Loading of Temozolomide and Curcumin into a Calix[4]arene-Based Nanocontainer for Potential Combined Chemotherapy: Binding Features, Enhanced Drug Solubility and Stability in Aqueous Medium. Nanomaterials 2021, 11, 2930. https://doi.org/10.3390/nano11112930
Migliore R, D’Antona N, Sgarlata C, Consoli GML. Co-Loading of Temozolomide and Curcumin into a Calix[4]arene-Based Nanocontainer for Potential Combined Chemotherapy: Binding Features, Enhanced Drug Solubility and Stability in Aqueous Medium. Nanomaterials. 2021; 11(11):2930. https://doi.org/10.3390/nano11112930
Chicago/Turabian StyleMigliore, Rossella, Nicola D’Antona, Carmelo Sgarlata, and Grazia M. L. Consoli. 2021. "Co-Loading of Temozolomide and Curcumin into a Calix[4]arene-Based Nanocontainer for Potential Combined Chemotherapy: Binding Features, Enhanced Drug Solubility and Stability in Aqueous Medium" Nanomaterials 11, no. 11: 2930. https://doi.org/10.3390/nano11112930