Prenatal Exposure to Heavy Metals Affects Gestational Age by Altering DNA Methylation Patterns
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Preparation
2.2. DNA Methylation Assay
2.3. Data Quality Control
2.4. Data Adjustment
2.5. Bioinformatics and Statistical Analyses
3. Results
3.1. Sample and Data Preparation
3.2. Assessment of Exposure
3.3. EWAS on Heavy Metal Exposures and Birth Outcomes
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Newborns (n = 367) | Mothers (of 367 Newborns) | ||
---|---|---|---|
Boys | 186 | Age (year) | 30.4 ± 3.6 |
Girls | 181 | BMI (kg/m2) | 22.9 ± 3.1 |
Gestational age (day) | 275.4 ± 8.2 | Smoker (yes/no) | 38/329 |
Preterm birth (case/control) | 5/362 | Parity (0/>0) | 209/158 |
Hg (μg/L) | 5.9 ± 2.8 | Hg (μg/L) | 3.6 ± 1.9 |
Pb (μg/L) | 10 ± 4.2 | Pb (μg/L) | 13.7 ± 5.7 |
Cd (μg/L) | 0.7 ± 0.2 | Cd (μg/L) | 1.6 ± 0.4 |
F-Statistics (p) | Pearson’s Coefficients | ||
---|---|---|---|
Maternal–Prenatal exposure | |||
Hg | 2.277 × 10−67 *** | 0.750 | |
Pb | 3.773 × 10−25 *** | 0.505 | |
Cd | 0.013 * | 0.130 | |
Exposure–Gestational age | |||
Maternal | Hg | 0.924 | 0.005 |
Pb | 0.199 | −0.067 | |
Cd | 0.303 | −0.054 | |
Prenatal | Hg | 0.542 | 0.032 |
Pb | 0.170 | −0.072 | |
Cd | 0.983 | −0.001 |
Differential Methylation (β) | Linear Regression | |||
---|---|---|---|---|
p-Value | q-Value | p-Value | Correlation | |
Maternal Cd | 8.6 × 10−6 | 0.14 | 1.07 × 10−5 | 0.2275 |
Gestational age | 5.4 × 10−10 | 2.7 × 10−7 | 6.4 × 10−10 | −0.3154 |
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Koh, E.J.; Yu, S.Y.; Kim, S.H.; Lee, J.S.; Hwang, S.Y. Prenatal Exposure to Heavy Metals Affects Gestational Age by Altering DNA Methylation Patterns. Nanomaterials 2021, 11, 2871. https://doi.org/10.3390/nano11112871
Koh EJ, Yu SY, Kim SH, Lee JS, Hwang SY. Prenatal Exposure to Heavy Metals Affects Gestational Age by Altering DNA Methylation Patterns. Nanomaterials. 2021; 11(11):2871. https://doi.org/10.3390/nano11112871
Chicago/Turabian StyleKoh, Eun Jung, So Yeon Yu, Seung Hwan Kim, Ji Su Lee, and Seung Yong Hwang. 2021. "Prenatal Exposure to Heavy Metals Affects Gestational Age by Altering DNA Methylation Patterns" Nanomaterials 11, no. 11: 2871. https://doi.org/10.3390/nano11112871