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Article

Sono-Assembly of the [Arg-Phe]4 Octapeptide into Biofunctional Nanoparticles

1
School of Chemistry, University of Melbourne, Melbourne, VIC 3010, Australia
2
School of Science, RMIT University, Melbourne, VIC 3000, Australia
3
Department of Chemical Sciences and Technologies, University of Rome “Tor Vergata”, 00133 Rome, Italy
4
Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Santo Andre 09210-580, Brazil
5
Rubber Technology Centre, Indian Institute of Technology, Kharagpur 721302, India
*
Authors to whom correspondence should be addressed.
Nanomaterials 2020, 10(9), 1772; https://doi.org/10.3390/nano10091772
Received: 13 August 2020 / Revised: 30 August 2020 / Accepted: 1 September 2020 / Published: 8 September 2020
High-frequency ultrasound treatment is found to be a one-pot green technique to produce peptide-based nanostructures by ultrasound assisted self-assembly of oligopeptides. [Arg-Phe]4 octapeptides, consisting of alternating arginine (Arg/R) and phenylalanine (Phe/F) sequences, were subjected to 430 kHz ultrasound in aqueous solution in the absence of any external agents, to form [RF]4 nanoparticles ([RF]4-NPs), ~220 nm in diameter. A comprehensive analysis of the obtained nanoparticles demonstrated that the aromatic moieties of the oligopeptides can undergo oxidative coupling to form multiple oligomeric species, which then self-assemble into well-defined fluorescent nanoparticles. [RF]4-NPs were functionalized with polyethylene glycol (PEGylated) to improve their colloidal stability. Unlike the parent peptide, the PEGylated [RF]4-NPs showed limited cytotoxicity towards MDA-MB-231 cells. Furthermore, the intracellular trafficking of PEGylated [RF]4-NPs was investigated after incubation with MDA-MB-231 cells to demonstrate their efficient endo-lysosomal escape. This work highlights that the combined use of ultrasonic technologies and peptides enables easy fabrication of nanoparticles, with potential application in drug delivery. View Full-Text
Keywords: [Arg-Phe]4 octapeptides; self-assembly; acoustic cavitation; sono-assembly; nanoparticles [Arg-Phe]4 octapeptides; self-assembly; acoustic cavitation; sono-assembly; nanoparticles
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MDPI and ACS Style

Baral, A.; Bhangu, S.K.; Cimino, R.; Pelin, J.N.B.D.; Alves, W.A.; Chattopadhyay, S.; Ashokkumar, M.; Cavalieri, F. Sono-Assembly of the [Arg-Phe]4 Octapeptide into Biofunctional Nanoparticles. Nanomaterials 2020, 10, 1772. https://doi.org/10.3390/nano10091772

AMA Style

Baral A, Bhangu SK, Cimino R, Pelin JNBD, Alves WA, Chattopadhyay S, Ashokkumar M, Cavalieri F. Sono-Assembly of the [Arg-Phe]4 Octapeptide into Biofunctional Nanoparticles. Nanomaterials. 2020; 10(9):1772. https://doi.org/10.3390/nano10091772

Chicago/Turabian Style

Baral, Anshul, Sukhvir K. Bhangu, Rita Cimino, Juliane N.B.D. Pelin, Wendel A. Alves, Santanu Chattopadhyay, Muthupandian Ashokkumar, and Francesca Cavalieri. 2020. "Sono-Assembly of the [Arg-Phe]4 Octapeptide into Biofunctional Nanoparticles" Nanomaterials 10, no. 9: 1772. https://doi.org/10.3390/nano10091772

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