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Sono-Assembly of the [Arg-Phe]4 Octapeptide into Biofunctional Nanoparticles

School of Chemistry, University of Melbourne, Melbourne, VIC 3010, Australia
School of Science, RMIT University, Melbourne, VIC 3000, Australia
Department of Chemical Sciences and Technologies, University of Rome “Tor Vergata”, 00133 Rome, Italy
Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Santo Andre 09210-580, Brazil
Rubber Technology Centre, Indian Institute of Technology, Kharagpur 721302, India
Authors to whom correspondence should be addressed.
Nanomaterials 2020, 10(9), 1772;
Received: 13 August 2020 / Revised: 30 August 2020 / Accepted: 1 September 2020 / Published: 8 September 2020
High-frequency ultrasound treatment is found to be a one-pot green technique to produce peptide-based nanostructures by ultrasound assisted self-assembly of oligopeptides. [Arg-Phe]4 octapeptides, consisting of alternating arginine (Arg/R) and phenylalanine (Phe/F) sequences, were subjected to 430 kHz ultrasound in aqueous solution in the absence of any external agents, to form [RF]4 nanoparticles ([RF]4-NPs), ~220 nm in diameter. A comprehensive analysis of the obtained nanoparticles demonstrated that the aromatic moieties of the oligopeptides can undergo oxidative coupling to form multiple oligomeric species, which then self-assemble into well-defined fluorescent nanoparticles. [RF]4-NPs were functionalized with polyethylene glycol (PEGylated) to improve their colloidal stability. Unlike the parent peptide, the PEGylated [RF]4-NPs showed limited cytotoxicity towards MDA-MB-231 cells. Furthermore, the intracellular trafficking of PEGylated [RF]4-NPs was investigated after incubation with MDA-MB-231 cells to demonstrate their efficient endo-lysosomal escape. This work highlights that the combined use of ultrasonic technologies and peptides enables easy fabrication of nanoparticles, with potential application in drug delivery. View Full-Text
Keywords: [Arg-Phe]4 octapeptides; self-assembly; acoustic cavitation; sono-assembly; nanoparticles [Arg-Phe]4 octapeptides; self-assembly; acoustic cavitation; sono-assembly; nanoparticles
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MDPI and ACS Style

Baral, A.; Bhangu, S.K.; Cimino, R.; Pelin, J.N.B.D.; Alves, W.A.; Chattopadhyay, S.; Ashokkumar, M.; Cavalieri, F. Sono-Assembly of the [Arg-Phe]4 Octapeptide into Biofunctional Nanoparticles. Nanomaterials 2020, 10, 1772.

AMA Style

Baral A, Bhangu SK, Cimino R, Pelin JNBD, Alves WA, Chattopadhyay S, Ashokkumar M, Cavalieri F. Sono-Assembly of the [Arg-Phe]4 Octapeptide into Biofunctional Nanoparticles. Nanomaterials. 2020; 10(9):1772.

Chicago/Turabian Style

Baral, Anshul, Sukhvir K. Bhangu, Rita Cimino, Juliane N.B.D. Pelin, Wendel A. Alves, Santanu Chattopadhyay, Muthupandian Ashokkumar, and Francesca Cavalieri. 2020. "Sono-Assembly of the [Arg-Phe]4 Octapeptide into Biofunctional Nanoparticles" Nanomaterials 10, no. 9: 1772.

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