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Open AccessArticle

XPS Modeling of Immobilized Recombinant Angiogenin and Apoliprotein A1 on Biodegradable Nanofibers

1
Research Institute of Clinical and Experimental Lymphology—Branch of the ICG SB RAS, 2 Timakova str., 630060 Novosibirsk, Russia
2
Laboratory for the Physics of Advanced Materials (LPM), Department of Physics and Materials Science, University of Luxembourg, L-1511 Luxembourg, Luxembourg
3
Institute of Biochemistry, FRC FTM 2 Timakova str., 630117 Novosibirsk, Russia
4
National University of Science and Technology “MISiS”, Leninsky pr. 4, 119049 Moscow, Russia
*
Author to whom correspondence should be addressed.
Nanomaterials 2020, 10(5), 879; https://doi.org/10.3390/nano10050879
Received: 15 March 2020 / Revised: 29 April 2020 / Accepted: 29 April 2020 / Published: 2 May 2020
The immobilization of viable proteins is an important step in engineering efficient scaffolds for regenerative medicine. For example, angiogenin, a vascular growth factor, can be considered a neurotrophic factor, influencing the neurogenesis, viability, and migration of neurons. Angiogenin shows an exceptional combination of angiogenic, neurotrophic, neuroprotective, antibacterial, and antioxidant activities. Therefore, this protein is a promising molecule that can be immobilized on carriers used for tissue engineering, particularly for diseases that are complicated by neurotrophic and vascular disorders. Another highly important and viable protein is apoliprotein A1. Nevertheless, the immobilization of these proteins onto promising biodegradable nanofibers has not been tested before. In this work, we carefully studied the immobilization of human recombinant angiogenin and apoliprotein A1 onto plasma-coated nanofibers. We developed a new methodology for the quantification of the protein density of these proteins using X-ray photoelectron spectroscopy (XPS) and modeled the XPS data for angiogenin and apoliprotein A1 (Apo-A1). These findings were also confirmed by the analysis of immobilized Apo-A1 using fluorescent microscopy. The presented methodology was validated by the analysis of fibronectin on the surface of plasma-coated poly(ε-caprolactone) (PCL) nanofibers. This methodology can be expanded for other proteins and it should help to quantify the density of proteins on surfaces using routine XPS data treatment. View Full-Text
Keywords: biotechnology; nanofibers; plasma; polymers; X-ray photoelectron spectroscopy; angiogenin biotechnology; nanofibers; plasma; polymers; X-ray photoelectron spectroscopy; angiogenin
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Manakhov, A.; Permyakova, E.; Ershov, S.; Miroshnichenko, S.; Pykhtina, M.; Beklemishev, A.; Kovalskii, A.; Solovieva, A. XPS Modeling of Immobilized Recombinant Angiogenin and Apoliprotein A1 on Biodegradable Nanofibers. Nanomaterials 2020, 10, 879.

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