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Open AccessArticle

Intercellular Trafficking of Gold Nanostars in Uveal Melanoma Cells for Plasmonic Photothermal Therapy

1
Departamento de Química Física, Universidad Complutense de Madrid, Avenida Complutense s/n, 28040 Madrid, Spain
2
Instituto de Investigación Sanitaria de Santiago (IDIS), Xerencia de Xestión Integrada de Santiago (XXIS/SERGAS), Hospital Clínico Universitario de Santiago, Travesía da Choupana s/n, 15706 Santiago de Compostela, Spain
3
Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), Avda. Córdoba s/n, 28041 Madrid, Spain
*
Authors to whom correspondence should be addressed.
Nanomaterials 2020, 10(3), 590; https://doi.org/10.3390/nano10030590 (registering DOI)
Received: 12 February 2020 / Revised: 4 March 2020 / Accepted: 11 March 2020 / Published: 24 March 2020
(This article belongs to the Special Issue Anti-Cancer, Biochemical and Immunological Activity of Nanoparticles )
Efficient plasmonic photothermal therapies (PPTTs) using non-harmful pulse laser irradiation at the near-infrared (NIR) are a highly sought goal in nanomedicine. These therapies rely on the use of plasmonic nanostructures to kill cancer cells while minimizing the applied laser power density. Cancer cells have an unsettled capacity to uptake, retain, release, and re-uptake gold nanoparticles, thus offering enormous versatility for research. In this work, we have studied such cell capabilities for nanoparticle trafficking and its impact on the effect of photothermal treatments. As our model system, we chose uveal (eye) melanoma cells, since laser-assisted eye surgery is routinely used to treat glaucoma and cataracts, or vision correction in refractive surgery. As nanostructure, we selected gold nanostars (Au NSs) due to their high photothermal efficiency at the near-infrared (NIR) region of the electromagnetic spectrum. We first investigated the photothermal effect on the basis of the dilution of Au NSs induced by cell division. Using this approach, we obtained high PPTT efficiency after several cell division cycles at an initial low Au NS concentration (pM regime). Subsequently, we evaluated the photothermal effect on account of cell division upon mixing Au NS-loaded and non-loaded cells. Upon such mixing, we observed trafficking of Au NSs between loaded and non-loaded cells, thus achieving effective PPTT after several division cycles under low irradiation conditions (below the maximum permissible exposure threshold of skin). Our study reveals the ability of uveal melanoma cells to release and re-uptake Au NSs that maintain their plasmonic photothermal properties throughout several cell division cycles and re-uptake. This approach may be readily extrapolated to real tissue and even to treat in situ the eye tumor itself. We believe that our method can potentially be used as co-therapy to disperse plasmonic gold nanostructures across affected tissues, thus increasing the effectiveness of classic PPTT. View Full-Text
Keywords: gold nanostars; nanoparticle endocytosis; nanoparticle exocytosis; femtosecond pulse laser; plasmonic photothermal therapy gold nanostars; nanoparticle endocytosis; nanoparticle exocytosis; femtosecond pulse laser; plasmonic photothermal therapy
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MDPI and ACS Style

Ahijado-Guzmán, R.; Sánchez-Arribas, N.; Martínez-Negro, M.; González-Rubio, G.; Santiago-Varela, M.; Pardo, M.; Piñeiro, A.; López-Montero, I.; Junquera, E.; Guerrero-Martínez, A. Intercellular Trafficking of Gold Nanostars in Uveal Melanoma Cells for Plasmonic Photothermal Therapy. Nanomaterials 2020, 10, 590.

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