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Open AccessArticle

Quantitative and Selective Surface Plasmon Resonance Response Based on a Reduced Graphene Oxide–Polyamidoamine Nanocomposite for Detection of Dengue Virus E-Proteins

1
Institute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400 UPM, Malaysia
2
Faculty of Science, Universiti Putra Malaysia, Serdang 43400 UPM, Malaysia
3
inLAZER Dynamics Sdn Bhd, InnoHub Unit, Putra Science Park, Universiti Putra Malaysia, Serdang 43400, Malaysia
4
Wireless and Photonics Network Research Centre, Faculty of Engineering, Universiti Putra Malaysia, Serdang 43400 UPM, Malaysia
*
Author to whom correspondence should be addressed.
Nanomaterials 2020, 10(3), 569; https://doi.org/10.3390/nano10030569
Received: 31 December 2019 / Revised: 31 January 2020 / Accepted: 11 February 2020 / Published: 21 March 2020
(This article belongs to the Special Issue Biosensors Based on Nanostructure Materials)
Dengue viral infection is one of the most common deadliest diseases and has become a recurrent issue for public health in tropical countries. Although the spectrum of clinical diagnosis and treatment have recently been established, the efficient and rapid detection of dengue virus (DENV) during viremia and the early febrile phase is still a great challenge. In this study, a dithiobis (succinimidyl undecanoate, DSU)/amine-functionalized reduced graphene oxide-–polyamidoamine dendrimer (DSU/amine-functionalized rGO–PAMAM) thin film-based surface plasmon resonance (SPR) sensor was developed for the detection of DENV 2 E-proteins. Different concentrations of DENV 2 E-proteins were successfully tested by the developed SPR sensor-based system. The performance of the developed sensor showed increased shift in the SPR angle, narrow full-width–half-maximum of the SPR curve, high detection accuracy, excellent figure of merit and signal-to-noise ratio, good sensitivity values in the range of 0.08–0.5 pM (S = 0.2576°/pM, R2 = 0.92), and a high equilibrium association constant (KA) of 7.6452 TM−1. The developed sensor also showed a sensitive and selective response towards DENV 2 E-proteins compared to DENV 1 E-proteins and ZIKV (Zika virus) E-proteins. Overall, it was concluded that the Au/DSU/amine-functionalized rGO–PAMAM thin film-based SPR sensor has potential to serve as a rapid clinical diagnostic tool for DENV infection. View Full-Text
Keywords: dengue virus; envelope proteins; graphene oxide; polyamidoamine; surface plasmon resonance dengue virus; envelope proteins; graphene oxide; polyamidoamine; surface plasmon resonance
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Omar, N.A.S.; Fen, Y.W.; Abdullah, J.; Sadrolhosseini, A.R.; Mustapha Kamil, Y.; Fauzi, N.‘.M.; Hashim, H.S.; Mahdi, M.A. Quantitative and Selective Surface Plasmon Resonance Response Based on a Reduced Graphene Oxide–Polyamidoamine Nanocomposite for Detection of Dengue Virus E-Proteins. Nanomaterials 2020, 10, 569.

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