Next Article in Journal
Modeling of Catalytic Centers Formation Processes during Annealing of Multilayer Nanosized Metal Films for Carbon Nanotubes Growth
Next Article in Special Issue
Novel Nanohybrids Based on Supramolecular Assemblies of Meso-tetrakis-(4-sulfonatophenyl) Porphyrin J-aggregates and Amine-Functionalized Carbon Nanotubes
Previous Article in Journal
Ultra-Broadband High-Efficiency Solar Absorber Based on Double-Size Cross-Shaped Refractory Metals
Previous Article in Special Issue
SERS Sensing Properties of New Graphene/Gold Nanocomposite
Open AccessArticle

Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution

1
LuMIn, CNRS, ENS Paris-Saclay, CentraleSupélec, Université Paris-Saclay, 91405 Orsay, France
2
Vectorologie et Thérapeutiques Anticancéreuses, CNRS, Institut Gustave Roussy, Université Paris-Saclay, 94805 Villejuif, France
3
SCBM, Institut Joliot, CEA, Université Paris-Saclay, 91191 Gif-sur-Yvette, France
4
Diamond Sensors Laboratory, Institut LIST, CEA, Université Paris-Saclay, 91191 Gif-sur-Yvette, France
5
Institut d’Alembert, CNRS, ENS Paris-Saclay, Université Paris-Saclay, 91190 Gif-sur-Yvette, France
*
Author to whom correspondence should be addressed.
Co-senior authors.
Nanomaterials 2020, 10(3), 553; https://doi.org/10.3390/nano10030553
Received: 7 February 2020 / Revised: 6 March 2020 / Accepted: 15 March 2020 / Published: 19 March 2020
(This article belongs to the Special Issue Carbon Nanomaterials for Therapy, Diagnosis, and Biosensing)
Nanodiamonds of detonation origin are promising delivery agents of anti-cancer therapeutic compounds in a whole organism like mouse, owing to their versatile surface chemistry and ultra-small 5 nm average primary size compatible with natural elimination routes. However, to date, little is known about tissue distribution, elimination pathways and efficacy of nanodiamonds-based therapy in mice. In this report, we studied the capacity of cationic hydrogenated detonation nanodiamonds to carry active small interfering RNA (siRNA) in a mice model of Ewing sarcoma, a bone cancer of young adults due in the vast majority to the EWS-FLI1 junction oncogene. Replacing hydrogen gas by its radioactive analog tritium gas led to the formation of labeled nanodiamonds and allowed us to investigate their distribution throughout mouse organs and their excretion in urine and feces. We also demonstrated that siRNA directed against EWS-FLI1 inhibited this oncogene expression in tumor xenografted on mice. This work is a significant step to establish cationic hydrogenated detonation nanodiamond as an effective agent for in vivo delivery of active siRNA. View Full-Text
Keywords: nanodiamond; tritium; biodistribution; Ewing sarcoma; drug delivery; siRNA; nanomedicine nanodiamond; tritium; biodistribution; Ewing sarcoma; drug delivery; siRNA; nanomedicine
Show Figures

Figure 1

MDPI and ACS Style

Claveau, S.; Nehlig, É.; Garcia-Argote, S.; Feuillastre, S.; Pieters, G.; Girard, H.A.; Arnault, J.-C.; Treussart, F.; Bertrand, J.-R. Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution. Nanomaterials 2020, 10, 553.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop