Surfactant-Free Synthesis and Scalable Purification of Triangular Gold Nanoprisms with Low Non-Specific Cellular Uptake

Ramírez-Jiménez, Rafael; Artiga, Álvaro; Mitchell, Scott G.; Martín-Rapún, Rafael; de la Fuente, Jesús M.

doi:10.3390/nano10030539

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Surfactant-Free Synthesis and Scalable Purification of Triangular Gold Nanoprisms with Low Non-Specific Cellular Uptake

Rafael Ramírez-Jiménez

^1,2,

Álvaro Artiga

^1,2

Scott G. Mitchell

^1,2

Rafael Martín-Rapún

^1,2,3,* and

Jesús M. de la Fuente

^1,2,*

Instituto de Ciencia de Materiales de Aragón (CSIC-Universidad de Zaragoza), c/ Pedro Cerbuna s/n, 50009 Zaragoza, Spain

Centro de Investigación Biomédica en Red in Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), 28029 Madrid, Spain

Instituto de Nanociencia de Aragón, Depto. Química Orgánica (Universidad de Zaragoza), c/ Mariano Esquillor s/n, 50018 Zaragoza, Spain

Authors to whom correspondence should be addressed.

Nanomaterials 2020, 10(3), 539; https://doi.org/10.3390/nano10030539

Received: 27 February 2020 / Revised: 10 March 2020 / Accepted: 11 March 2020 / Published: 17 March 2020

(This article belongs to the Section Synthesis, Interfaces and Nanostructures)

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Abstract

Gold nanoprisms possess remarkable optical properties that make them useful for medical biotechnology applications such as diagnosis and photothermal therapy. However, shape-selective synthesis of gold nanoprisms is not trivial and typically requires either toxic surfactants or time-consuming purification protocols, which can limit their applicability. Here, we show how triangular gold nanoprisms of different sizes can be purified by precipitation using the non-toxic glutathione ligand, thereby removing the need for toxic surfactants and bottleneck purification techniques. The protocol is amenable for direct scaling up as no instrumentation is required in the critical purification step. The new purification method provides a two-fold increased yield in gold nanoprisms compared to electrophoretic filtration, while providing nanoprisms of similar localized surface plasmon resonance wavelength. Crucially, the gold nanoprisms isolated using this methodology show fewer non-specific interactions with cells and lower cellular internalization, which paves the way for a higher selectivity in therapeutic applications. View Full-Text

Keywords: gold nanoparticles; gold nanoprisms; gold nanoplates; localized surface plasmon resonance; non-specific cellular uptake; plasmonic nanoparticles; photothermal therapy

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MDPI and ACS Style

Ramírez-Jiménez, R.; Artiga, Á.; Mitchell, S.G.; Martín-Rapún, R.; de la Fuente, J.M. Surfactant-Free Synthesis and Scalable Purification of Triangular Gold Nanoprisms with Low Non-Specific Cellular Uptake. Nanomaterials 2020, 10, 539. https://doi.org/10.3390/nano10030539

AMA Style

Ramírez-Jiménez R, Artiga Á, Mitchell SG, Martín-Rapún R, de la Fuente JM. Surfactant-Free Synthesis and Scalable Purification of Triangular Gold Nanoprisms with Low Non-Specific Cellular Uptake. Nanomaterials. 2020; 10(3):539. https://doi.org/10.3390/nano10030539

Chicago/Turabian Style

Ramírez-Jiménez, Rafael, Álvaro Artiga, Scott G. Mitchell, Rafael Martín-Rapún, and Jesús M. de la Fuente. 2020. "Surfactant-Free Synthesis and Scalable Purification of Triangular Gold Nanoprisms with Low Non-Specific Cellular Uptake" Nanomaterials 10, no. 3: 539. https://doi.org/10.3390/nano10030539

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Surfactant-Free Synthesis and Scalable Purification of Triangular Gold Nanoprisms with Low Non-Specific Cellular Uptake

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