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A Sensitive FRET Biosensor Based on Carbon Dots-Modified Nanoporous Membrane for 8-hydroxy-2′-Deoxyguanosine (8-OHdG) Detection with [email protected] Nanoparticles as Signal Quenchers

by Weiwei Ye 1,2,*, Yu Zhang 3, Wei Hu 3, Liwen Wang 3, Yu Zhang 4 and Ping Wang 5
1
Key Laboratory of E&M, MOE, Zhejiang University of Technology, Hangzhou 310023, China
2
College of Mechanical Engineering, Zhejiang University of Technology, Hangzhou 310023, China
3
Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China
4
Mechanical and Automotive Engineering, School of Engineering, RMIT University, Melbourne, Victoria 3004, Australia
5
Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China
*
Author to whom correspondence should be addressed.
Nanomaterials 2020, 10(10), 2044; https://doi.org/10.3390/nano10102044
Received: 4 September 2020 / Revised: 30 September 2020 / Accepted: 2 October 2020 / Published: 16 October 2020
A sensitive fluorescence resonance energy transfer (FRET) biosensor is proposed to detect 8-hydroxy-2′-deoxyguanosine (8-OHdG), which is a typical DNA oxidation damage product excreted in human urine. The FRET biosensor was based on carbon dots (CDs)-modified nanoporous alumina membrane with CDs as fluorescence donors. Gold nanoparticles were encapsulated in zeolitic imidazolate framework-8 to form [email protected] nanoparticles as signal quenchers. CDs and [email protected] nanoparticles were biofunctionalized by 8-OHdG antibody. The capture of 8-OHdG on the membrane substrates can bring [email protected] nanoparticles closely to CDs. With 350 nm excitation, the fluorescence of CDs was quenched by [email protected] nanoparticles and FRET effect occurred. The quenching efficiency was analyzed. The limit of detection (LOD) was 0.31 nM. Interference experiments of the FRET biosensor showed good specificity for 8-OHdG detection. The biosensor could detect urinary 8-OHdG sensitively and selectively with simple sample pretreatment processes. It shows applicability for detecting biomarkers of DNA damage in urine or other biological fluids. View Full-Text
Keywords: nanoporous alumina membrane; fluorescence resonance energy transfer; [email protected] nanoparticles; DNA oxidation damage; 8-OHdG detection nanoporous alumina membrane; fluorescence resonance energy transfer; [email protected] nanoparticles; DNA oxidation damage; 8-OHdG detection
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Ye, W.; Zhang, Y.; Hu, W.; Wang, L.; Zhang, Y.; Wang, P. A Sensitive FRET Biosensor Based on Carbon Dots-Modified Nanoporous Membrane for 8-hydroxy-2′-Deoxyguanosine (8-OHdG) Detection with [email protected] Nanoparticles as Signal Quenchers. Nanomaterials 2020, 10, 2044.

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