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Open AccessArticle

Competitive Surface Colonization of Antibacterial and Bioactive Materials Doped with Strontium and/or Silver Ions

1
Department of Health Science Università del Piemonte Orientale UPO, 28100 Novara, Italy
2
Interdisciplinary Research Center of Autoimmune Diseases, Center for Translational Research on Autoimmune and Allergic Diseases—CAAD, 28100 Novara, Italy
3
Department of Applied Science and Technology, Politecnico di Torino, 10129 Torino, Italy
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Interdipartimental Laboratory PolitoBIOMedLab, Politecnico di Torino, 10129 Torino, Italy
5
Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, 1200 Matsumoto, Kasugai, Aichi 487-8501, Japan
*
Author to whom correspondence should be addressed.
Nanomaterials 2020, 10(1), 120; https://doi.org/10.3390/nano10010120 (registering DOI)
Received: 18 November 2019 / Revised: 28 December 2019 / Accepted: 6 January 2020 / Published: 8 January 2020
(This article belongs to the Section Biology and Medicines)
Nowadays, there is a large amount of research aimed at improving the multifunctional behavior of the biomaterials for bone contact, including the concomitant ability to induce apatite formation (bioactivity), fast and effective osteoblasts colonization, and antibacterial activity. The aim of this study is to develop antibacterial and bioactive surfaces (Ti6Al4V alloy and a silica-based bioactive glass) by chemical doping with strontium and/or silver ions. The surfaces were characterized by Scanning Electron Microscopy equipped with Energy Dispersive X ray Spectroscopy (SEM-EDS), X-ray photoelectron spectroscopy (XPS), and Transmission Electron Microscopy (TEM). To better focus on the cells–bacteria competition for the implant surface, in addition to the standard assays for the evaluation of the bacteria adhesion (ISO22196) and for single-cell cultures or biofilm formation, an innovative set of co-cultures of cells and bacteria is here proposed to simulate a competitive surface colonization. The results suggest that all the bioactive tested materials were cytocompatible toward the bone progenitor cells representative for the self-healing process, and that the doped ones were effective in reducing the surface colonization from a pathogenic drug-resistant strain of Staphylococcus aureus. The co-cultures experiments demonstrated that the doped surfaces were able to protect the adhered osteoblasts from the bacteria colonization as well as prevent the infection prior to the surface colonization by the osteoblasts. View Full-Text
Keywords: antibacterial activity; cytotoxicity; silver; strontium; competition for the surface; bone antibacterial activity; cytotoxicity; silver; strontium; competition for the surface; bone
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MDPI and ACS Style

Cochis, A.; Barberi, J.; Ferraris, S.; Miola, M.; Rimondini, L.; Vernè, E.; Yamaguchi, S.; Spriano, S. Competitive Surface Colonization of Antibacterial and Bioactive Materials Doped with Strontium and/or Silver Ions. Nanomaterials 2020, 10, 120.

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