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Covalent Immobilization of β-Glucosidase into Mesoporous Silica Nanoparticles from Anhydrous Acetone Enhances Its Catalytic Performance

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Department of Agricultural Sciences, Università degli Studi di Napoli Federico II, Via Università 100, 80055 Portici (Na), Italy
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Department of Chemical Engineering, Materials and Industrial Production, Università degli Studi di Napoli Federico II, P.le Tecchio 80, 80125 Napoli, Italy
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Department of Chemical Science, Università degli Studi di Napoli Federico II, Complesso Universitario di Monte S. Angelo via Cintia, 80126 Napoli, Italy
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Istituto Motori-CNR, via G. Marconi 4, 80125 Napoli, Italy
*
Author to whom correspondence should be addressed.
Nanomaterials 2020, 10(1), 108; https://doi.org/10.3390/nano10010108
Received: 19 November 2019 / Revised: 10 December 2019 / Accepted: 30 December 2019 / Published: 5 January 2020
(This article belongs to the Section Energy and Catalysis)
An immobilization protocol of a model enzyme into silica nanoparticles was applied. This protocol exploited the use of the bifunctional molecule triethoxysilylpropylisocyanate (TEPI) for covalent binding through a linker of suitable length. The enzyme β-glucosidase (BG) was anchored onto wrinkled silica nanoparticles (WSNs). BG represents a bottleneck in the conversion of lignocellulosic biomass into biofuels through cellulose hydrolysis and fermentation. The key aspect of the procedure was the use of an organic solvent (anhydrous acetone) in which the enzyme was not soluble. This aimed to restrict its conformational changes and thus preserve its native structure. This approach led to a biocatalyst with improved thermal stability, characterized by high immobilization efficiency and yield. It was found that the apparent KM value was about half of that of the free enzyme. The Vmax was about the same than that of the free enzyme. The biocatalyst showed a high operational stability, losing only 30% of its activity after seven reuses. View Full-Text
Keywords: covalent immobilization; β-glucosidase; mesoporous silica nanoparticles covalent immobilization; β-glucosidase; mesoporous silica nanoparticles
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MDPI and ACS Style

Sannino, F.; Costantini, A.; Ruffo, F.; Aronne, A.; Venezia, V.; Califano, V. Covalent Immobilization of β-Glucosidase into Mesoporous Silica Nanoparticles from Anhydrous Acetone Enhances Its Catalytic Performance. Nanomaterials 2020, 10, 108.

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