Next Article in Journal
Nail Properties and Bone Health: A Review
Previous Article in Journal
Novel Chlorhexidine-Loaded Polymeric Nanoparticles for Root Canal Treatment
Open AccessArticle

Growing Neural PC-12 Cell on Crosslinked Silica Aerogels Increases Neurite Extension in the Presence of an Electric Field

1
Department of Physics and Materials Science, University of Memphis, Memphis, TN 38152, USA
2
Department of Biological Sciences, University of Memphis, Memphis, TN 38152, USA
*
Author to whom correspondence should be addressed.
J. Funct. Biomater. 2018, 9(2), 30; https://doi.org/10.3390/jfb9020030
Received: 15 February 2018 / Revised: 11 April 2018 / Accepted: 18 April 2018 / Published: 20 April 2018
Externally applied electrical stimulation (ES) has been shown to enhance the nerve regeneration process and to influence the directionality of neurite outgrowth. In addition, the physical and chemical properties of the substrate used for nerve-cell regeneration is critical in fostering regeneration. Previously, we have shown that polyurea-crosslinked silica aerogels (PCSA) exert a positive influence on the extension of neurites by PC-12 cells, a cell-line model widely used to study neurite extension and electrical excitability. In this work, we have examined how an externally applied electric field (EF) influences the extension of neurites in PC-12 cells grown on two substrates: collagen-coated dishes versus collagen-coated crosslinked silica aerogels. The externally applied direct current (DC) bias was applied in vitro using a custom-designed chamber containing polydimethysiloxane (PDMS) embedded copper electrodes to create an electric field across the substrate for the cultured PC-12 cells. Results suggest orientation preference towards the anode, and, on average, longer neurites in the presence of the applied DC bias than with 0 V DC bias. In addition, neurite length was increased in cells grown on silica-crosslinked aerogel when compared to cells grown on regular petri-dishes. These results further support the notion that PCSA is a promising material for nerve regeneration. View Full-Text
Keywords: aerogel; porous; scaffold; substrate; PC-12; neuron; electrical stimulation; electric field; guidance aerogel; porous; scaffold; substrate; PC-12; neuron; electrical stimulation; electric field; guidance
Show Figures

Figure 1

MDPI and ACS Style

Lynch, K.J.; Skalli, O.; Sabri, F. Growing Neural PC-12 Cell on Crosslinked Silica Aerogels Increases Neurite Extension in the Presence of an Electric Field. J. Funct. Biomater. 2018, 9, 30.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop