Effects of Medium and Temperature on Cellular Responses in the Superficial Zone of Hypo-Osmotically Challenged Articular Cartilage
Abstract
:1. Introduction
Component | DMEM 290 mOsm | PBS 290 mOsm |
---|---|---|
Amino Acids | × | – |
Vitamins | × | – |
D-glucose (Dextrose) | × | – |
Sodium Chloride (NaCl) | 6,400 mg/L | 8,000 mg/L |
Potassium Chloride (KCl) | 400 mg/L | 200 mg/L |
Sodium Phosphate monobasic (NaH2PO4) | 141 mg/L | – |
Sodium Phosphate dibasic (Na2HPO4) | – | 1,440 mg/L |
Potassium Phosphate monobasic (KH2PO4) | – | 240 mg/L |
Calcium Chloride (CaCl2) anhyd. | 264 mg/L | – |
Ferric Nitrate (Fe(NO3)3''9H2O) | 0.1 mg/L | – |
Magnesium Sulfate (MgSO4) anhyd. | 200 mg/L | – |
Sodium Bicarbonate (NaHCO3) | 3,700 mg/L | – |
2. Experimental Section
2.1. Sample Preparation
2.2. Confocal Laser Scanning Microscopy
2.3. Data Analysis
2.4. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Acknowledgments
References
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Huttu, M.; Turunen, S.; Sokolinski, V.; Tiitu, V.; Lammi, M.; Korhonen, R.K. Effects of Medium and Temperature on Cellular Responses in the Superficial Zone of Hypo-Osmotically Challenged Articular Cartilage. J. Funct. Biomater. 2012, 3, 544-555. https://doi.org/10.3390/jfb3030544
Huttu M, Turunen S, Sokolinski V, Tiitu V, Lammi M, Korhonen RK. Effects of Medium and Temperature on Cellular Responses in the Superficial Zone of Hypo-Osmotically Challenged Articular Cartilage. Journal of Functional Biomaterials. 2012; 3(3):544-555. https://doi.org/10.3390/jfb3030544
Chicago/Turabian StyleHuttu, Mari, Siru Turunen, Viktoria Sokolinski, Virpi Tiitu, Mikko Lammi, and Rami K. Korhonen. 2012. "Effects of Medium and Temperature on Cellular Responses in the Superficial Zone of Hypo-Osmotically Challenged Articular Cartilage" Journal of Functional Biomaterials 3, no. 3: 544-555. https://doi.org/10.3390/jfb3030544