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Open AccessArticle

Electrospun Scaffolds Containing Silver-Doped Hydroxyapatite with Antimicrobial Properties for Applications in Orthopedic and Dental Bone Surgery

1
School of Clinical Dentistry, University of Sheffield, Shefield 0114, UK
2
Beijing Laboratory of Biomedical Materials, Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Beijing 100083, China
3
Beijing Key Laboratory of Micro-nano Energy and Sensor, Beijing institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing 100083, China
4
Central Laboratory, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, China
5
Certara, Simcyp division, Sheffield 0114, UK
*
Authors to whom correspondence should be addressed.
These authors have contributed equally to the work.
J. Funct. Biomater. 2020, 11(3), 58; https://doi.org/10.3390/jfb11030058
Received: 21 May 2020 / Revised: 9 July 2020 / Accepted: 28 July 2020 / Published: 14 August 2020
(This article belongs to the Special Issue Bacterial Interactions with Dental and Medical Materials)
Preventing the development of osteomyelitis while enhancing bone regeneration is challenging, with relatively little progress to date in translating promising technologies to the clinic. Nanoscale hydroxyapatite (nHA) has been employed as a bone graft substitute, and recent work has shown that it may be modified with silver to introduce antimicrobial activity against known pathogens. The aim of this study was to incorporate silver-doped nHA into electrospun scaffolds for applications in bone repair. Silver-doped nHA was produced using a modified, rapid mixing, wet precipitation method at 2, 5, 10 mol.% silver. The silver-doped nHA was added at 20 wt.% to a polycaprolactone solution for electrospinning. Bacteria studies demonstrated reduced bacterial presence, with Escherichia coli and Staphylococcus aureus undetectable after 96 h of exposure. Mesenchymal stem cells (MSCs) were used to study both toxicity and osteogenicity of the scaffolds using PrestoBlue® and alkaline phosphatase (ALP) assays. Innovative silver nHA scaffolds significantly reduced E. coli and S. aureus bacterial populations while maintaining cytocompatibility with mammalian cells and enhancing the differentiation of MSCs into osteoblasts. It was concluded that silver-doped nHA containing scaffolds have the potential to act as an antimicrobial device while supporting bone tissue healing for applications in orthopedic and dental bone surgery. View Full-Text
Keywords: electrospinning; antimicrobial; nano-hydroxyapatite; silver; toxicity; bone regeneration electrospinning; antimicrobial; nano-hydroxyapatite; silver; toxicity; bone regeneration
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MDPI and ACS Style

Paterson, T.E.; Shi, R.; Tian, J.; Harrison, C.J.; De Sousa Mendes, M.; Hatton, P.V.; Li, Z.; Ortega, I. Electrospun Scaffolds Containing Silver-Doped Hydroxyapatite with Antimicrobial Properties for Applications in Orthopedic and Dental Bone Surgery. J. Funct. Biomater. 2020, 11, 58.

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