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Anti-Photoaging Effect of Plant Extract Fermented with Lactobacillus buchneri on CCD-986sk Fibroblasts and HaCaT Keratinocytes

1
Department of Pharmacology, College of Korean Medicine, Sangji University, Gangwon-do 26339, Korea
2
Department of Korean Ophthalmology and Otolaryngology and Dermatology, College of Korean Medicine, Sangji University, Wonju, Gangwon 26339, Korea
3
Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Iksan, Jeonbuk 59338, Korea
4
WonNature, Wonkwang University, Iksan, Jeonbuk 54538, Korea
5
Research Institute, Wonkwang herb Co., Ltd., Jinan, Jeonbuk 55442, Korea
*
Author to whom correspondence should be addressed.
J. Funct. Biomater. 2020, 11(1), 3; https://doi.org/10.3390/jfb11010003
Received: 29 November 2019 / Revised: 4 January 2020 / Accepted: 7 January 2020 / Published: 9 January 2020
Ultraviolet (UV) exposure triggers the abnormal production of reactive oxygen (ROS) species and the expression of matrix metalloproteinases (MMPs) that are responsible for photoaging. Probiotics are widely used in healthcare and for immune enhancement. One probiotic, Lactobacillus buchneri is found in Kimchi. This study was aimed at assessing the anti-photoaging effect of plant extracts fermented with L. buchneri (PELB) to develop functional cosmetics. We investigated the anti-photoaging effect of PELB in a UVB-induced photoaging in vitro model and selected effective extracts using the elastase inhibition assay, ELISA for Type I procollagen and collagenase-1, and quantitative real time PCR. Normal human dermal fibroblasts and epidermal keratinocytes were pre-treated with PELB and exposed to UVB. We found that PELB decreased elastase activity and increased type I collagen expression in a UVB-induced photoaging in vitro model. In addition, PELB greatly reduced collagenase activity and MMP mRNA levels in a UVB-induced photoaging in vitro model. Furthermore, PELB promoted the expression of moisture factor and anti-oxidant enzymes in a UVB-induced photoaging in vitro model. These results indicated that the PELB could be potential candidates for the protective effects against UVB-induced photoaging. Overall, these results suggest that PELB might be useful natural components of cosmetic products.
Keywords: Lactobacillus buchneri; photoaging; UVB; metalloproteinases; reactive oxygen species Lactobacillus buchneri; photoaging; UVB; metalloproteinases; reactive oxygen species
MDPI and ACS Style

Kang, Y.-M.; Hong, C.-H.; Kang, S.-H.; Seo, D.-S.; Kim, S.-O.; Lee, H.-Y.; Sim, H.-J.; An, H.-J. Anti-Photoaging Effect of Plant Extract Fermented with Lactobacillus buchneri on CCD-986sk Fibroblasts and HaCaT Keratinocytes. J. Funct. Biomater. 2020, 11, 3.

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