Enhanced Osteogenic Differentiation of Human Fetal Cartilage Rudiment Cells on Graphene Oxide-PLGA Hybrid Microparticles

Thickett, Stuart C.; Hamilton, Ella; Yogeswaran, Gokulan; Zetterlund, Per B.; Farrugia, Brooke L.; Lord, Megan S.

doi:10.3390/jfb10030033

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Enhanced Osteogenic Differentiation of Human Fetal Cartilage Rudiment Cells on Graphene Oxide-PLGA Hybrid Microparticles

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School of Natural Sciences (Chemistry), University of Tasmania, Hobart, TAS 7001, Australia

Graduate School of Biomedical Engineering, UNSW Sydney, Sydney, NSW 2052, Australia

Centre for Advanced Macromolecular Design, School of Chemical Engineering, UNSW Sydney, Sydney, NSW 2052, Australia

Authors to whom correspondence should be addressed.

J. Funct. Biomater. 2019, 10(3), 33; https://doi.org/10.3390/jfb10030033

Received: 24 May 2019 / Revised: 15 July 2019 / Accepted: 25 July 2019 / Published: 30 July 2019

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Abstract

Poly(d,l–lactide–co–glycolide) (PLGA) has been extensively explored for bone regeneration applications; however, its clinical use is limited by low osteointegration. Therefore, approaches that incorporate osteoconductive molecules are of great interest. Graphene oxide (GO) is gaining popularity for biomedical applications due to its ability to bind biological molecules and present them for enhanced bioactivity. This study reports the preparation of PLGA microparticles via Pickering emulsification using GO as the sole surfactant, which resulted in hybrid microparticles in the size range of 1.1 to 2.4 µm based on the ratio of GO to PLGA in the reaction. Furthermore, this study demonstrated that the hybrid GO-PLGA microparticles were not cytotoxic to either primary human fetal cartilage rudiment cells or the human osteoblast-like cell line, Saos-2. Additionally, the GO-PLGA microparticles promoted the osteogenic differentiation of the human fetal cartilage rudiment cells in the absence of exogenous growth factors to a greater extent than PLGA alone. These findings demonstrate that GO-PLGA microparticles are cytocompatible, osteoinductive and have potential as substrates for bone tissue engineering. View Full-Text

Keywords: PLGA; graphene oxide; osteoblast; stem cell; bone tissue engineering

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MDPI and ACS Style

Thickett, S.C.; Hamilton, E.; Yogeswaran, G.; Zetterlund, P.B.; Farrugia, B.L.; Lord, M.S. Enhanced Osteogenic Differentiation of Human Fetal Cartilage Rudiment Cells on Graphene Oxide-PLGA Hybrid Microparticles. J. Funct. Biomater. 2019, 10, 33. https://doi.org/10.3390/jfb10030033

AMA Style

Thickett SC, Hamilton E, Yogeswaran G, Zetterlund PB, Farrugia BL, Lord MS. Enhanced Osteogenic Differentiation of Human Fetal Cartilage Rudiment Cells on Graphene Oxide-PLGA Hybrid Microparticles. Journal of Functional Biomaterials. 2019; 10(3):33. https://doi.org/10.3390/jfb10030033

Chicago/Turabian Style

Thickett, Stuart C., Ella Hamilton, Gokulan Yogeswaran, Per B. Zetterlund, Brooke L. Farrugia, and Megan S. Lord. 2019. "Enhanced Osteogenic Differentiation of Human Fetal Cartilage Rudiment Cells on Graphene Oxide-PLGA Hybrid Microparticles" Journal of Functional Biomaterials 10, no. 3: 33. https://doi.org/10.3390/jfb10030033

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Enhanced Osteogenic Differentiation of Human Fetal Cartilage Rudiment Cells on Graphene Oxide-PLGA Hybrid Microparticles

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