Effect of Chestnut Tannins and Vitamin E Supplementation to Linseed Oil-Enriched Diets on Growth Performance, Meat Quality, and Intestinal Morphology of Broiler Chickens
Round 1
Reviewer 1 Report
The manuscript discusses the effect of Chestnut Tannins and Vitamin E supplementation to Linseed Oil-Enriched diets on growth performance, meat quality, and intestinal morphology of broiler chickens.
Some parts of the manuscript can be improved so it can be easier to understand and follow.
L46-55: irrelevant to the topic of the manuscript.
The discussion has to be revised, some points need to be calcified such as how these (Vitamin E) additives improved breast yield and dressing percentages.
Also, how Tannis improved the histological parameters, and what is the significant of these improvements if it was not reflected on performance.
Also, what is the relation between vitamin E and Tannis, I don't see any relation between the treatments.
Author Response
Dear reviewer,
thank you for your valuable comments and suggestions. I hope that we managed to correct the manuscript according to your instructions.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
Peric and colleagues investigate the effects of adding chestnut tannins and vitamin E to linseed oil-enriched diets on broiler growth performance, meat quality, and intestinal morphology. Though many studies have been conducted on the supplementation of vitamin E in linseed oil-enriched diet in broiler chickens, few studies have focused on the addition of tannin in linseed oil-enriched diet. This gives some scientific merit to this manuscript. Overall, this manuscript is a well-written and well-executed study. Thus, I recommend accepted with minor changes. The following are some of the minor issues.
- Please double-check the affiliation standard format. I see repeated addresses in the manuscript.
- The introduction is well-written. However, the authors could potentially improve the introduction by emphasizing the cost-effectiveness of using these two supplements to improve broiler production. This would be beneficial to the readers, particularly those working in the poultry feed industry.
- Methodology; Line 95; On what basis did the author decide to use tannins at a concentration of 500 mg/kg? Perhaps the authors could provide a couple of lines of explanation in the introductory part.
- There was no negative control in this study (without the addition of linseed oil). Could the authors please comment on this?
- Line 268; change "this is line" to "this is in line"
- Conclusion; Can authors conclude whether tannin or vitamin E is beneficial to poultry production? This work found that a linseed oil-enriched diet can improve broiler growth performance on its own.
Author Response
Dear reviewer,
thank you for your valuable comments and suggestions. I hope that we managed to correct the manuscript according to your instructions.
Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report
I don't see any thing related 129-132.
Point 4: What is the relation between vitamin E and Tannis, I don't see any relation between the treatments.
Response 4: During the planning of the experiment the idea was to examine the effect of tannins as a potential antioxidant which could be added in the oil-enriched feed. In that context we considered the oil enriched group without additives as negative control, and the vitamin E group as a sort of positive control, since the antioxidative capacity of vitamin E has been proven. Additional sentence is added in lines 129-132.
Also, the discussion can be improve.
Author Response
Dear reviewer,
thank you for your comment. We hope that changes that we made in the manuscript according to your suggestions, have improved the discussion .
Author Response File: Author Response.pdf
