Morphological Characterization and Determination of Aflatoxigenic and Non-Aflatoxigenic Aspergillus flavus Isolated from Sweet Corn Kernels and Soil in Malaysia
Abstract
:1. Introduction
2. Materials and Methods
2.1. Description of the Study Site
2.2. Sample Collection
2.2.1. Sweet Corn Kernels
2.2.2. Soil Sampling
2.3. Isolation and Identification of Aspergillus flavus
2.3.1. Preparation of Growth Media
2.3.2. Aspergillus flavus from Sweet Corn Samples
2.3.3. Aspergillus flavus from Soil Samples
2.3.4. Morphological Confirmation of Aspergillus flavus
2.4. Determination of Aflatoxigenic Potential of Aspergillus flavus
3. Results
3.1. Detection of Aspergillus flavus
3.2. Morphological Characterization of Aspergillus flavus
3.2.1. Macroscopic Characteristics of Aspergillus flavus on PDA
3.2.2. Macroscopic Characteristics of Aspergillus flavus on DRBC
3.2.3. Macroscopic Characteristics of Aspergillus flavus on AFPA
3.3. Microscopic Characteristics of Aspergillus flavus
3.4. Screening for Aflatoxin Production
4. Discussion
4.1. Prevalence of Aspergillus flavus across Sweet Corn Farms in Malaysia
4.2. Morphological Characterization of Aspergillus flavus
4.3. Discrimination between Aflatoxigenic and Non-aflatoxigenic Aspergillus flavus
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Isolate | No. | CCA |
---|---|---|
AKR1 | 1 | + |
AKR2 | 2 | - |
AKR3 | 3 | + |
AKR4 | 4 | + |
AKR5 | 5 | + |
AKR6 | 6 | - |
AKR7 | 7 | - |
AKR8 | 8 | + |
AKR9 | 9 | + |
AKR10 | 10 | - |
AKR11 | 11 | - |
AKR12 | 12 | - |
ARV13 | 13 | - |
ARV14 | 14 | + |
ARV15 | 15 | - |
ARV16 | 16 | - |
ARV17 | 17 | + |
ARV18 | 18 | + |
ARV19 | 19 | - |
ARV20 | 20 | - |
ARV21 | 21 | - |
ARV22 | 22 | - |
AK23 | 23 | + |
AK24 | 24 | + |
AK25 | 25 | - |
AK26 | 26 | - |
AK27 | 27 | - |
AK28 | 28 | + |
AK29 | 29 | - |
AK30 | 30 | - |
AKL31 | 31 | + |
AKL32 | 32 | - |
AKL33 | 33 | - |
AKL34 | 34 | + |
AKL35 | 35 | + |
AKL36 | 36 | - |
AKL37 | 37 | + |
AKL38 | 38 | + |
AKL39 | 39 | - |
AKL40 | 40 | + |
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Khan, R.; Mohamad Ghazali, F.; Mahyudin, N.A.; Samsudin, N.I.P. Morphological Characterization and Determination of Aflatoxigenic and Non-Aflatoxigenic Aspergillus flavus Isolated from Sweet Corn Kernels and Soil in Malaysia. Agriculture 2020, 10, 450. https://doi.org/10.3390/agriculture10100450
Khan R, Mohamad Ghazali F, Mahyudin NA, Samsudin NIP. Morphological Characterization and Determination of Aflatoxigenic and Non-Aflatoxigenic Aspergillus flavus Isolated from Sweet Corn Kernels and Soil in Malaysia. Agriculture. 2020; 10(10):450. https://doi.org/10.3390/agriculture10100450
Chicago/Turabian StyleKhan, Rahim, Farinazleen Mohamad Ghazali, Nor Ainy Mahyudin, and Nik Iskandar Putra Samsudin. 2020. "Morphological Characterization and Determination of Aflatoxigenic and Non-Aflatoxigenic Aspergillus flavus Isolated from Sweet Corn Kernels and Soil in Malaysia" Agriculture 10, no. 10: 450. https://doi.org/10.3390/agriculture10100450
APA StyleKhan, R., Mohamad Ghazali, F., Mahyudin, N. A., & Samsudin, N. I. P. (2020). Morphological Characterization and Determination of Aflatoxigenic and Non-Aflatoxigenic Aspergillus flavus Isolated from Sweet Corn Kernels and Soil in Malaysia. Agriculture, 10(10), 450. https://doi.org/10.3390/agriculture10100450