Both HCV Infection and Elevated Liver Stiffness Significantly Impacts on Several Parameters of T-Cells Homeostasis in HIV-Infected Patients
Abstract
:1. Introduction
2. Experimental Section
2.1. Study Population
2.2. Cell Samples
2.3. Immunophenotypic Analysis
2.4. Statistical Analysis
3. Results
3.1. Characteristics of Patients Included in the Study
3.2. HCV Co-Infection Significantly Impacts on T-Cells Homeostasis
3.3. The Degree of Liver Stiffness Significantly Impacts on T-Cells Homeostasis
3.4. HCV Eradication with DAAs Does Not Completely Revert T-Cells Homeostasis Disturbances
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Characteristic | HIV Group (n = 25) | HIV/HCV Group (n = 25) | p-Value |
---|---|---|---|
Age (years) | 48 (42–55) | 44 (39–48) | 0.11 |
Gender (% of males) | 88% | 100% | 0.24 |
Time since HIV diagnosis (years) | 9 (6–14) | 7 (2–10) | 0.07 |
Time since HCV diagnosis (years) | NA | 2 (1.5–5) | NA |
Time on ART (years) | 5 (3.5–7.5) | 4 (2–9) | 0.63 |
CD4 count (cells/μL) | 816 (605–992) | 735 (577–902) | 0.31 |
Ratio CD4/CD8 | 0.84 (0.56–1.34) | 0.75 (0.60–1.09) | 0.49 |
ALT level (IU/L) | 32 (24–37) | 74 (49–162) | <0.0001 |
AST level (IU/L) | 27 (22–31) | 58 (37–122) | <0.0001 |
GGT level (IU/L) | 36 (22–54) | 55 (25–119) | 0.06 |
Total cholesterol level (mg/dL) | 194 (162–227) | 157 (125–177) | <0.0001 |
HDL level (mg/dL) | 42 (37–50) | 46 (33–50) | 0.815 |
LDL level (mg/dL) | 119 (93–146) | 85 (71–104) | 0.001 |
HCV-RNA (log copies/mL) | NA | 6.1 (5.8–6.4) | NA |
HIV transmission route (%) | 1 | ||
Sexual | 100% | 96% | |
Parenteral | 0% | 4% | |
HCV genotype (%) | NA | ||
1a | NA | 60% | |
1b | NA | 12% | |
4 | NA | 28% | |
Liver stiffness (measured by elastography) (%) | NA | ||
<7.1 KPa | NA | 60% | |
≥7.1 KPa | NA | 40% | |
DAA regimen | |||
GZR/EBR | NA | 64% | |
SOF/LDV | NA | 32% | |
OBV/PTV/r/DSV | NA | 4% |
T-Cell Subsets | Level of T-Cell Subsets Median [IQR] | p-Value * | |
---|---|---|---|
pre-DAAs HIV/HCV Coinfected Patients | HIV Monoinfected Patients | ||
CD4+CD45RA+CCR7-PD1-TIM3+ | 0.91 (0.45–1.57) | 0.46 (0.27–0.74) | 0.076 |
CD4+CD45RA+CCR7-CD38+HLADR- | 5.59 (3.84–8.83) | 3.29 (2.19–0.47) | 0.004 |
CD8+CD45RA+CCR7-PD1+TIM3+ | 0.59 (0.31–1.25) | 0.29 (0.18–0.44) | 0.006 |
CD8+CD45RA+CCR7-CD38+HLADR- | 1.41 (1.01–3.66) | 0.67 (0.39–0.91) | <0.0001 |
T-Cell Subsets | Level of T-Cell Subsets Median [IQR] | p-Value * | |
---|---|---|---|
post-DAAs HIV/HCV Coinfected Patients | HIV Monoinfected Patients | ||
CD8+CD45RA+CCR7-PD1+TIM3+ | 0.51 (0.23–0.99) | 0.29 (0.18–0.44) | 0.051 |
CD8+CD45RA+CCR7-CD38+HLADR- | 1.20 (0.77–2.51) | 0.67 (0.39–1.58) | 0.003 |
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Restrepo, C.; Álvarez, B.; Valencia, J.L.; García, M.; Navarrete-Muñoz, M.A.; Ligos, J.M.; Cabello, A.; Prieto, L.; Nistal, S.; Montoya, M.; et al. Both HCV Infection and Elevated Liver Stiffness Significantly Impacts on Several Parameters of T-Cells Homeostasis in HIV-Infected Patients. J. Clin. Med. 2020, 9, 2978. https://doi.org/10.3390/jcm9092978
Restrepo C, Álvarez B, Valencia JL, García M, Navarrete-Muñoz MA, Ligos JM, Cabello A, Prieto L, Nistal S, Montoya M, et al. Both HCV Infection and Elevated Liver Stiffness Significantly Impacts on Several Parameters of T-Cells Homeostasis in HIV-Infected Patients. Journal of Clinical Medicine. 2020; 9(9):2978. https://doi.org/10.3390/jcm9092978
Chicago/Turabian StyleRestrepo, Clara, Beatriz Álvarez, José L Valencia, Marcial García, María A Navarrete-Muñoz, José M Ligos, Alfonso Cabello, Laura Prieto, Sara Nistal, María Montoya, and et al. 2020. "Both HCV Infection and Elevated Liver Stiffness Significantly Impacts on Several Parameters of T-Cells Homeostasis in HIV-Infected Patients" Journal of Clinical Medicine 9, no. 9: 2978. https://doi.org/10.3390/jcm9092978