Melatonin Improves Mitochondrial Dynamics and Function in the Kidney of Zücker Diabetic Fatty Rats
Abstract
:1. Introduction
2. Experimental Section
2.1. Chemicals
2.2. Animals and Experimental Protocols
2.3. Experimental Protocol
2.4. Clearance Studies: Measurement of Urinary and Serum Parameters
2.5. Mitochondria Preparation
2.6. Western Blotting for Mitochondrial Dynamic Proteins
2.7. Mitochondrial Respirometry
2.8. Spectrophotometric Assay of Respiratory Chain Complex
2.9. Determination of Mitochondrial Nitrites
2.10. Mitochondrial Activity of SOD
2.11. Statistical Analysis
3. Results
3.1. Renal Function Assessment
3.2. Western Blotting Drp1, Mfn2, and OPA1 Expression Results
3.3. Mitochondrial Respirometry
3.4. Mitochondrial Complex IV Activity Results
3.5. Mitochondrial Oxidative Status
3.5.1. Nitrite Levels
3.5.2. Superoxide Dismutase Activity
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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ZL-C | ZL-M | ZDF-C | ZDF-M | |
---|---|---|---|---|
Total urine volume (mL/day) | 10.3 ± 1.3 | 10.4 ± 1.3 | 85.8 ± 6 ### | 55.9 ± 8 *** |
Proteinuria (mg/day) | 4.8 ± 0.9 | 4.9 ± 0.8 | 94 ± 18 ### | 67 ± 8 *** |
Creatinine clearance (mL/min) | 3.2 ± 0.1 | 3.2 ± 0.2 | 2.5 ± 0.2 ### | 2.9 ± 0.2 * |
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Agil, A.; Chayah, M.; Visiedo, L.; Navarro-Alarcon, M.; Rodríguez Ferrer, J.M.; Tassi, M.; Reiter, R.J.; Fernández-Vázquez, G. Melatonin Improves Mitochondrial Dynamics and Function in the Kidney of Zücker Diabetic Fatty Rats. J. Clin. Med. 2020, 9, 2916. https://doi.org/10.3390/jcm9092916
Agil A, Chayah M, Visiedo L, Navarro-Alarcon M, Rodríguez Ferrer JM, Tassi M, Reiter RJ, Fernández-Vázquez G. Melatonin Improves Mitochondrial Dynamics and Function in the Kidney of Zücker Diabetic Fatty Rats. Journal of Clinical Medicine. 2020; 9(9):2916. https://doi.org/10.3390/jcm9092916
Chicago/Turabian StyleAgil, Ahmad, Meriem Chayah, Lucia Visiedo, Miguel Navarro-Alarcon, José Manuel Rodríguez Ferrer, Mohamed Tassi, Russel J. Reiter, and Gumersindo Fernández-Vázquez. 2020. "Melatonin Improves Mitochondrial Dynamics and Function in the Kidney of Zücker Diabetic Fatty Rats" Journal of Clinical Medicine 9, no. 9: 2916. https://doi.org/10.3390/jcm9092916