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Article

Metabolic Activity of Human Embryos after Thawing Differs in Atmosphere with Different Oxygen Concentrations

1
Department of Obstetrics and Gynecology, Faculty of Medicine, Masaryk University and University Hospital Brno, 602 00 Brno, Czech Republic
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Department of Veterinary Sciences, Czech University of Life Sciences in Prague, 165 00 Prague, Czech Republic
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Department of Biochemistry, Faculty of Science, Masaryk University, 602 00 Brno, Czech Republic
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Department of Nursing and Midwifery, Faculty of Medicine, Masaryk University, 602 00 Brno, Czech Republic
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Department of Veterinary Surgery, Nicolaus Copernicus University, 87 100 Torun, Poland
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Department of Histology and Embryology, Poznan University of Medical Sciences, 61 701 Poznan, Poland
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Department of Anatomy, Poznan University of Medical Sciences, 61 701 Poznan, Poland
*
Author to whom correspondence should be addressed.
J. Clin. Med. 2020, 9(8), 2609; https://doi.org/10.3390/jcm9082609
Received: 10 July 2020 / Revised: 6 August 2020 / Accepted: 10 August 2020 / Published: 12 August 2020
(This article belongs to the Section Obstetrics & Gynecology)
The vitrification of human embryos is more and more frequently being utilized as a method of assisted reproduction. For this technique, gentle treatment of the embryos after thawing is crucial. In this study, the balance of amino acids released to/consumed from the cultivation media surrounding the warmed embryos was observed in the context of a cultivation environment, which was with the atmospheric oxygen concentration ≈20% or with a regulated oxygen level—hysiological (5%). It is the first time that total amino acid turnover in human embryos after their freezing at post compaction stages has been evaluated. During this study, progressive embryos (developed to blastocyst stage) and stagnant embryos (without developmental progression) were analyzed. It was observed that the embryos cultivated in conditions of physiological oxygen levels (5% oxygen) showed a significantly lower consumption of amino acids from the cultivation media. Progressively developing embryos also had significantly lower total amino acid turnovers (consumption and production of amino acids) when cultured in conditions with physiological oxygen levels. Based on these results it seems that a cultivation environment with a reduced oxygen concentration decreases the risk of degenerative changes in the embryos after thawing. Therefore, the cultivation of thawed embryos in an environment with physiological oxygen levels may preclude embryonal stagnation, and can support the further development of human embryos after their thawing. View Full-Text
Keywords: amino acids; human embryo; in vitro cultivation; capillary electrophoresis; fluorescence detection; metabolic activity amino acids; human embryo; in vitro cultivation; capillary electrophoresis; fluorescence detection; metabolic activity
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MDPI and ACS Style

Ješeta, M.; Celá, A.; Žáková, J.; Mádr, A.; Crha, I.; Glatz, Z.; Kempisty, B.; Ventruba, P. Metabolic Activity of Human Embryos after Thawing Differs in Atmosphere with Different Oxygen Concentrations. J. Clin. Med. 2020, 9, 2609. https://doi.org/10.3390/jcm9082609

AMA Style

Ješeta M, Celá A, Žáková J, Mádr A, Crha I, Glatz Z, Kempisty B, Ventruba P. Metabolic Activity of Human Embryos after Thawing Differs in Atmosphere with Different Oxygen Concentrations. Journal of Clinical Medicine. 2020; 9(8):2609. https://doi.org/10.3390/jcm9082609

Chicago/Turabian Style

Ješeta, Michal, Andrea Celá, Jana Žáková, Aleš Mádr, Igor Crha, Zdeněk Glatz, Bartosz Kempisty, and Pavel Ventruba. 2020. "Metabolic Activity of Human Embryos after Thawing Differs in Atmosphere with Different Oxygen Concentrations" Journal of Clinical Medicine 9, no. 8: 2609. https://doi.org/10.3390/jcm9082609

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